Improving the Differentiation of human pluripotent stem cells to beta-cells.
2012-04
Loading...
View/Download File
Persistent link to this item
Statistics
View StatisticsJournal Title
Journal ISSN
Volume Title
Title
Improving the Differentiation of human pluripotent stem cells to beta-cells.
Authors
Published Date
2012-04
Publisher
Type
Thesis or Dissertation
Abstract
Human pluripotent stem cells (hPSCs) have the ability to differentiate into any cell type within the body, thus holding the potential for treating the beta-cell loss in type 1 diabetes through a cell replacement therapy. Though effective protocols have been produced for creating beta-cells from hPSCs, the efficiency of this process can be improved to yield more beta-cells. Here, we hypothesized that the addition of the hormones prolactin and human growth hormone to our established differentiation protocol will result in more beta-cells or beta-cell progenitors. This is based on work with isolated pancreatic islets, which showed that these hormones are able to stimulate proliferation of mature beta-cells and increase islet volume. And as much of the work with these hormones has been in rodent islets, we also present data showing that prolactin is able to stimulate cell division and islet growth in human islets. Any influences of the hormones were observed through gene expression analysis and a cell-death assay. This work will inform future work on creating beta-cells from hPSCs, and hopefully move the potential therapy closer to the clinic.
Keywords
Description
University of Minnesota M.S. thesis. April 2012. Major: Stem cell biology. Advisor: Meri Firpo. 1 computer file (PDF); iv, 71 pages.
Related to
Replaces
License
Series/Report Number
Funding information
Isbn identifier
Doi identifier
Previously Published Citation
Suggested citation
Dalton, Joseph Patrick. (2012). Improving the Differentiation of human pluripotent stem cells to beta-cells.. Retrieved from the University Digital Conservancy, https://hdl.handle.net/11299/125281.
Content distributed via the University Digital Conservancy may be subject to additional license and use restrictions applied by the depositor. By using these files, users agree to the Terms of Use. Materials in the UDC may contain content that is disturbing and/or harmful. For more information, please see our statement on harmful content in digital repositories.