Minisatellites in meiosis: crossover regulation and stability of repetitive DNA.
2012-04
Loading...
View/Download File
Persistent link to this item
Statistics
View StatisticsJournal Title
Journal ISSN
Volume Title
Title
Minisatellites in meiosis: crossover regulation and stability of repetitive DNA.
Authors
Published Date
2012-04
Publisher
Type
Thesis or Dissertation
Abstract
The minisatellite associated with the human HRAS1 proto-oncogene has an enhancer effect on HRAS1 expression. Rare minisatellite alleles have stronger enhancer activity and are frequently found in primary tumors of cancer patients. Rare alleles derive from a common allele that has undergone an alteration in length; alterations occur primarily during the process of meiotic recombination. Identifying factors that regulate minisatellite stability during meiosis is important to our understanding of the initiation and predisposition of minisatellite-associated human diseases. This is the focus of the Kirkpatrick lab using a minisatellite model system in yeast Saccharomyces cerevisiae where an HRAS1 minisatellite allele has been inserted into the promoter region upstream of the HIS4 locus on chromosome III. In this study, we identified CSM3, TOF1, and MRC1 as factors that contribute to meiotic minisatellite alterations. We also uncovered a novel recombination phenotype associated with the HRAS1 minisatellite in MSH4 and MSH5 mutants. Both of these projects have broader implications on our understanding of factors that regulate minisatellite alterations during meiosis. We present models based on our data and previously published research to explain the observed phenotypes.
Description
University of Minnesota Ph.D. dissertation. April 2012. Major: Molecular, Cellular, Developmental Biology and Genetics. Advisor: Dr. David Kirkpatrick. 1 computer file (PDF); v, 137 pages.
Related to
Replaces
License
Collections
Series/Report Number
Funding information
Isbn identifier
Doi identifier
Previously Published Citation
Suggested citation
LeClere, Andrea Ruth. (2012). Minisatellites in meiosis: crossover regulation and stability of repetitive DNA.. Retrieved from the University Digital Conservancy, https://hdl.handle.net/11299/126297.
Content distributed via the University Digital Conservancy may be subject to additional license and use restrictions applied by the depositor. By using these files, users agree to the Terms of Use. Materials in the UDC may contain content that is disturbing and/or harmful. For more information, please see our statement on harmful content in digital repositories.