Identification of Extracellular Matrix Proteins Supportive of Endothelial Differentiation
2018-12
Loading...
View/Download File
Persistent link to this item
Statistics
View StatisticsJournal Title
Journal ISSN
Volume Title
Title
Identification of Extracellular Matrix Proteins Supportive of Endothelial Differentiation
Authors
Published Date
2018-12
Publisher
Type
Thesis or Dissertation
Abstract
Efficient differentiation of pluripotent stem cells into endothelial cells represents one potential means of resolving vascularization issue in the field of tissue engineering. Recently, extracellular matrix proteins (ECM) have been shown potent stimulators of differentiation, including endothelial differentiation. Unclear is how the ECM can exhibit varying signaling stimuli over the course of a 14-day differentiation time scale. Here we test the possibility that an ECM deposited by cells over time could provide the temporal cues needed for endothelial differentiation. Mass spectrometry was employed as a means of measuring changes in deposition of ECM by cells and remodeling with ECM-triggered endothelial differentiation. As a start, the workflow for attaining accurate quantification was optimized. Then temporal dynamics of ECM expression were represented as a heat map and linear graphs of those ECM proteins with unique temporal shifts relative to control cultures. Collagen XVIII, Fibrilin 2, Fibulin 1, Galectin 9, Laminin subunit 5, Nidogen 2 fulfill these criteria and support the possibility that endothelial differentiation spurred by exogenously provided ECM is brought to completion by an evolving ECM composite.
Description
University of Minnesota M.S. thesis.December 2018. Major: Stem Cell Biology. Advisor: Brenda Ogle. 1 computer file (PDF); vii, 126 pages.
Related to
Replaces
License
Series/Report Number
Funding information
Isbn identifier
Doi identifier
Previously Published Citation
Suggested citation
Ahmadova, Sevinj. (2018). Identification of Extracellular Matrix Proteins Supportive of Endothelial Differentiation. Retrieved from the University Digital Conservancy, https://hdl.handle.net/11299/218670.
Content distributed via the University Digital Conservancy may be subject to additional license and use restrictions applied by the depositor. By using these files, users agree to the Terms of Use. Materials in the UDC may contain content that is disturbing and/or harmful. For more information, please see our statement on harmful content in digital repositories.