Characterizing mVenus adsorption to photodegraded polyethylene using circular dichroism and fluorescence spectroscopy
2022-08
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Characterizing mVenus adsorption to photodegraded polyethylene using circular dichroism and fluorescence spectroscopy
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2022-08
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Due to their versatility and relative cost-effectiveness, plastics as a material have gained increasing popularity and are heavily utilized by almost every major industry in the modern day. Their exponential rate of production coupled with a lack of proper disposal methods, however, have resulted in the global environmental issue of plastic pollution. Upon entering the ecosystem, plastic surfaces can act as a foundation for the formation of microbial communities known as biofilms. An initial key step to biofilm growth is the attachment of bacterial surface proteins onto the polymer. In this study, we examine structural changes of a “hard” model protein in the presence of environmentally relevant plastics. Using the intrinsic probes of the mVenus protein, a model yellow fluorescent protein (YFP), we study its structural response to variably photo-aged polyethylene (PE) through circular dichroism (CD) and tryptophan (W)/YFP-fluorescence spectroscopy. Upon binding to aged PE, mVenus undergoes mild secondary structure rearrangement. Interestingly, a forbidden transition in W-fluorescence is observed, evolving from the interaction between the sole tryptophan in mVenus and the increasingly hydrophilic surface of PE as the polymer is progressively photo-oxidized. The beta barrel and beta sheet structure of mVenus retains the overall stability of the protein, whereas the local structure and turn regions accommodate the protein-polymer interactions based on polymer surface chemistry. We can therefore start to predict that proteins bind variably during the initial docking of cells as the secondary structure behaves distinctly based on the age of the film to which it attaches. The dependence of protein docking on the extent of PE-irradiation reveals that film age, polymer type, and structural stability can either accelerate or inhibit biofilm growth.
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University of Minnesota M.S. thesis. 2022. Major: Chemistry. Advisor: Melissa Maurer-Jones. 1 computer file (PDF); 83 pages.
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Amaris, Althea. (2022). Characterizing mVenus adsorption to photodegraded polyethylene using circular dichroism and fluorescence spectroscopy. Retrieved from the University Digital Conservancy, https://hdl.handle.net/11299/250027.
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