High-Throughput Method for Microfluidic Placement of Cells in Micropatterned Tissues

Sevcik, Emily
2013-04-20
Thumbnail Image

View/Download File

Sevcik.pdf (501.16 KB)

Persistent link to this item

https://hdl.handle.net/11299/150247
Statistics
View Statistics

Journal Title

Journal ISSN

Volume Title

Title

High-Throughput Method for Microfluidic Placement of Cells in Micropatterned Tissues

Authors

Published Date

2013-04-20

Publisher

Type

Presentation

Abstract

Recent studies have shown that cell shape and tissue structure can dictate functional behavior in engineered tissues (1). One method for controlling tissue structure in vitro is microcontact printing, where extracellular matrix proteins are patterned on a substrate to construct arrays of single cells or multicellular tissues. This technique is used to create tissues that mimic in vivo architecture which can be used to study tissue properties and disease mechanisms (2). Traditional seeding of cells on the substrate is imprecise, but our group has developed a microfluidic device for spatial control of cell seeding, which creates more replicable high-fidelity tissues. However, the current method is low-throughput and labor intensive. Here, we present a scalable system of multiple microfluidic devices for parallel cell seeding. This high-throughput, precise approach reduces experimental variation, making biochemical assays on single cell arrays possible in future work. We will use this system to create large arrays of single cells of various shapes for phenotypic studies and to create arrays of tissues with varying cellular organization. 1)Alford, P. W., Nesmith, A. P., Seywerd, J. N., Grosberg, A., & Parker, K. K. (2011). Vascular smooth muscle contractility depends on cell shape. Integrative Biology, 3(11), 1063-1070. 2)Ruiz, S. A., & Chen, C. S. (2007). Microcontact printing: A tool to pattern. Soft Matter, 3(2), 168-177.

Keywords

Description

This report describes a high throughput microfluidic method to control cell placement in two-dimensional tissue engineering. Other contributors include Zaw Win and Patrick W. Alford (Faculty Mentor).

Related to

Replaces

License

Collections

UMTC Undergraduate Research Presentations and Papers (UROP)

Series/Report Number

Funding information

This research was supported by the Undergraduate Research Opportunities Program (UROP).

Isbn identifier

Doi identifier

Previously Published Citation

Suggested citation

Sevcik, Emily. (2013). High-Throughput Method for Microfluidic Placement of Cells in Micropatterned Tissues. Retrieved from the University Digital Conservancy, https://hdl.handle.net/11299/150247.

Content distributed via the University Digital Conservancy may be subject to additional license and use restrictions applied by the depositor. By using these files, users agree to the Terms of Use. Materials in the UDC may contain content that is disturbing and/or harmful. For more information, please see our statement on harmful content in digital repositories.