Isolation and Characterization of Novel Hyperthermophilic Manganese (II)-oxidase from Purple Pool Yellowstone National Park (2021-03-12)
Loading...
View/Download File
Persistent link to this item
Statistics
View StatisticsJournal Title
Journal ISSN
Volume Title
Title
Isolation and Characterization of Novel Hyperthermophilic Manganese (II)-oxidase from Purple Pool Yellowstone National Park (2021-03-12)
Published Date
2021
Publisher
Type
Other
Abstract
Manganese (Mn) oxides are some of the strongest naturally occurring oxides in nature,playing an important role in geochemical cycling, and living systems. Several theories onthe role of Mn in microbial systems exist, but there is not enough supporting evidence toprovide a strong argument for these theories. Understanding the role of Mn in microbialsystems will allow for further investigations into the evolution of early life, defensivemechanisms, and electron transfer systems in microbes. Preliminary sequence data ofbiofilms and enrichments of biofilms collected from a Mn-depositing hot spring (102ÂșC) inYellow Stone National Park (YNP), have yielded findings that suggest a novelhyperthermophilic archaea may be responsible for the accumulation of Mn(III/IV) oxideswithin the hot spring. There are currently no known hyperthermophilic archaea that iscapable of oxidizing Mn(II), an investigation into the properties of the Mn oxidizingproperties will have wide impacts in fields of early life, bioremediation, and industry. Theinvestigation will involve biofilm studies, to study its role in Mn oxidation mechanisms, andalso in Mn oxide morphologies as a respect of Mn concentration and flow rate. Thesestudies would be useful in biomarker analysis in Mn containing geological features, mostnotably in YNP. A bioinformatics component of the investigation will involve aninvestigation of the genome and mapping of possible gene sequences responsible for Mnoxidation. The Mn oxidase genes will then be further contextualized by constructingmetabolic pathways of the genomes. The biochemical component will include the isolationand expression, and synthesis of the proteins from the sequenced identified for Mnoxidation. As a protein originating from hyperthermophilic archaea, it is hypothesized that itwill have retained activity in high temperatures. These hypothesis will be tested by runningactivity assays, in respect to product/intermediate formations. In addition, denaturationconditions will be tested to examine the durability of the protein structure. Characterizationof the novel Mn oxidation components of the thermophilic archaea will be impactful infields of environmental science, bioinorganic chemistry, and industry.
Description
Friday, March 12, 2021; 3:30 p.m. Remote Via Zoom; Naoto Tozaki, Master's Student, Department of Chemistry & Biochemistry, University of Minnesota Duluth; Research Advisor's [sic]: Dr. Steve Barry and Dr. Wendy Smythe
Related to
Replaces
License
Collections
Series/Report Number
Funding information
Isbn identifier
Doi identifier
Previously Published Citation
Suggested citation
Tozaki, Naoto; University of Minnesota Duluth. Department of Chemistry and Biochemistry. (2021). Isolation and Characterization of Novel Hyperthermophilic Manganese (II)-oxidase from Purple Pool Yellowstone National Park (2021-03-12). Retrieved from the University Digital Conservancy, https://hdl.handle.net/11299/220632.
Content distributed via the University Digital Conservancy may be subject to additional license and use restrictions applied by the depositor. By using these files, users agree to the Terms of Use. Materials in the UDC may contain content that is disturbing and/or harmful. For more information, please see our statement on harmful content in digital repositories.