A Collagen Droplet Platform For Screening Malignant Peripheral Nerve Sheath Tumors

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A Collagen Droplet Platform For Screening Malignant Peripheral Nerve Sheath Tumors

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2022-06

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The services of monolayer culture in probing underlying mechanisms around cancer cell biology are extensive and have resulted in novel findings that are too numerous to reference in this paper alone. Rather, broadly speaking, 2D has provided a cornerstone in understanding and exploration of high-throughput pharmacological screens, cellular level imaging, methods of studying metastasis, response to chemotherapy agents, and a great deal more. However, recent research in the field of cancer biology demands us to consider the TME, and to supplement current 2D models by migrating towards 3D methodologies. Improved development of 3D platforms and their further integration into the realm of cancer biology is vital for continued scientific progress of the field. Herein, we present the advancement and novel application of a microfluidic-based 3D collagen droplet platform for utilization with ex vivo PDX MPNSTs. The platform is then utilized for a single and dual drug screen to probe for potential future therapeutic agents based on cellular viability over time. We culminate these studies by presenting a repository of ex vivo PDX MPNSTs across multiple research organizations.

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University of Minnesota M.S. thesis. June 2022. Major: Biomedical Engineering. Advisor: David Wood. 1 computer file (PDF); v, 33 pages.

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Conniff, Eric. (2022). A Collagen Droplet Platform For Screening Malignant Peripheral Nerve Sheath Tumors. Retrieved from the University Digital Conservancy, https://hdl.handle.net/11299/258855.

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