The Effects of Radiation on Marrow Cell Differentiation

Persistent link to this item

Statistics
View Statistics

Journal Title

Journal ISSN

Volume Title

Title

The Effects of Radiation on Marrow Cell Differentiation

Published Date

2009-04-08

Publisher

Type

Presentation

Abstract

With improved patient survival, cancer treatment induced bone loss (CTIBL) has become and increasingly serious complication of therapy. This study will be looking at the effect of radiation on a marrow cell line's potential to differentiate into osteoblastic, adipocytic, or fibroblastic cells. Alterations in cell differentiation may play a role in CITBL. Marrow stromal cells harvested from p53 knock out transgenic mice were plated into culture flasks. Twelve hours after plating the flasks were irradiated at different clinically relevant radiation doses. The flasks were fixed and stained following two weeks, four weeks, and six weeks of culture and separated into three different analytical groups looking at: Mineralization, Adiposity, and Gene Expression between the different sets of radiation doses. We are investigating the effects of radiation, time dependent cellular differentiation, and reductions in mineralization and adiposity. Preliminary data suggests an inverse relationship between osteoblastic and adipogenic differentiation by marrow cells in vitro following increasing doses of gamma irradiation. This may aid in understanding some fundamental mechanisms of Cancer-Treatment Induced Bone Loss.

Description

Additional contributors: Susanta Hui (faculty mentor) and Louis Kidder (faculty mentor).

Related to

Replaces

License

Series/Report Number

Funding information

This project was supported by: the Undergraduate Research Opportunities Program (UROP); the National Institute of Child Health and Human Development (NICHD) grant number 1K12-HD055887-01; NIH grant R03 AR055333-02; The Academic Health Center; Grant in Aid; and a Masonic Cancer Center grant.

Isbn identifier

Doi identifier

Previously Published Citation

Suggested citation

Coghill, Kathleen. (2009). The Effects of Radiation on Marrow Cell Differentiation. Retrieved from the University Digital Conservancy, https://hdl.handle.net/11299/50136.

Content distributed via the University Digital Conservancy may be subject to additional license and use restrictions applied by the depositor. By using these files, users agree to the Terms of Use. Materials in the UDC may contain content that is disturbing and/or harmful. For more information, please see our statement on harmful content in digital repositories.