Investigating the Possible Role of a Glycosyl Transferase Protein in the Biosynthesis of Long-Chain Hydrocarbons in Shewanella oneidensis

Loading...
Thumbnail Image

Persistent link to this item

Statistics
View Statistics

Journal Title

Journal ISSN

Volume Title

Title

Investigating the Possible Role of a Glycosyl Transferase Protein in the Biosynthesis of Long-Chain Hydrocarbons in Shewanella oneidensis

Published Date

2012-04-18

Publisher

Type

Presentation

Abstract

In the search for alternative sources of energy, new organisms are being looked at as potential biofuel producers. It has been shown that the longchain hydrocarbons produced by the bacterium Shewanella oneidensis can be broken down into usable fuel. It is known that the enzyme OleA is largely responsible for hydrocarbon synthesis, but other proteins may also play a role. In my thesis work, I deleted the gene SO_3174, which encodes a glycosyl transferase protein and was found to be interrupted in a transposon screen for increased hydrocarbon production, from S. oneidensis MR-1. I then tried to show that deleting SO_3174 resulted in an increase in hydrocarbon production just as the interruption of the gene had. The deletion strain showed an increased fluorescence in the presence of Nile Red dye, a hydrophobic dye that can be used to indirectly detect hydrocarbon levels. However, the deletion strain did not exhibit increased hydrocarbons during direct analysis of nonpolar extractions. These same results were obtained from a strain containing both the SO_3174 deletion and Stenotrophomonas maltophilia OleA. Overall, I found that the protein encoded by SO_3174 most likely does not play a role in hydrocarbon biosynthesis in S. oneidensis.

Description

Mentor: Jeffrey Gralnick

Related to

Replaces

License

Series/Report Number

Funding information

This research was supported by the Undergraduate Research Opportunities Program (UROP).

Isbn identifier

Doi identifier

Previously Published Citation

Suggested citation

Bergquist, Carl. (2012). Investigating the Possible Role of a Glycosyl Transferase Protein in the Biosynthesis of Long-Chain Hydrocarbons in Shewanella oneidensis. Retrieved from the University Digital Conservancy, https://hdl.handle.net/11299/123131.

Content distributed via the University Digital Conservancy may be subject to additional license and use restrictions applied by the depositor. By using these files, users agree to the Terms of Use. Materials in the UDC may contain content that is disturbing and/or harmful. For more information, please see our statement on harmful content in digital repositories.