Post-Transcriptionally Modified tRNAs for Enhanced Translation

Prasad, Angelin
Kalb, Evan
Adamala, Kate
2024-04
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Post-Transcriptionally Modified tRNAs for Enhanced Translation

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2024-04

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Synthesizing peptides from non-natural amino acids presents challenges such as maintaining a correct reading frame and proper tRNA charging. Flexizyme aids tRNA charging with non-natural amino acids, often using in-vitro transcribed tRNAs. In-vivo transcribed tRNAs, with post-transcriptional modifications, hypothetically offer better stability. Stability for tRNAfMET was assessed via Tm values, indicating higher stability in in-vivo transcribed tRNAs. However, Hibit luminescence assays showed no significant translation efficiency difference between in-vitro and in-vivo transcribed tRNAs. In conclusion, post-transcriptional modifications may not significantly enhance flexizyme reactions. Future steps involve identifying limiting factors in the system or tRNAs.

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Poster presentation for Undergraduate Research Symposium on April 18th, 2024. Poster presentation was on Post-Transcriptionally Modified tRNAs for enhanced translation. Faculty advisor: Kate Adamala

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This research was supported by the Undergraduate Research Opportunities Program (UROP).

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Prasad, Angelin; Kalb, Evan; Adamala, Kate. (2024). Post-Transcriptionally Modified tRNAs for Enhanced Translation. Retrieved from the University Digital Conservancy, https://hdl.handle.net/11299/262393.

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