Between Dec 19, 2024 and Jan 2, 2025, datasets can be submitted to DRUM but will not be processed until after the break. Staff will not be available to answer email during this period, and will not be able to provide DOIs until after Jan 2. If you are in need of a DOI during this period, consider Dryad or OpenICPSR. Submission responses to the UDC may also be delayed during this time.
 

Communication in Membrane Repair

Loading...
Thumbnail Image

Persistent link to this item

Statistics
View Statistics

Journal Title

Journal ISSN

Volume Title

Title

Communication in Membrane Repair

Published Date

2015-06

Publisher

Type

Thesis or Dissertation

Abstract

The force generated by muscle cells places a high amount of stress on their plasma membranes creating lesions which must be effectively repaired in order for the cell to survive. Multiple proteins have been implicated in the membrane repair process, one of which is dysferlin, a seven C2 domain containing protein. Of the seven C2 domains within dysferlin, only the C2A domain exists in two isoforms and has been suggested to be the Ca2+ sensor within dysferlin. Mutations within dysferlin have been found to cause several types of muscular dystrophies including Limb-Girdle muscular dystrophy, Myoshi Myopathy and Distal Anterior Compartment Myopathy. In vivo studies have revealed that after membrane rupture, dysferlin interacts with multiple proteins including annexin A2. In order to gain a better understanding of how this system functions, this author used methods including differential scanning calorimetry (DSC) and spectroscopy (fluorescence and circular dichroism) to examine both isoforms of the C2A domain and annexin A2. All three proteins were found to be marginally stable suggestive of a system that is highly capable of information propagation. From this, a picture emerges where mutations within dysferlin could result in a dramatic shift in the conformational ensemble available to the protein, which would interfere with its ability to properly interact and communicate with the other members of the membrane repair machinery. This would result in the loss of the ability to properly repair the membrane.

Description

University of Minnesota M.S. thesis. June 2015. Major: Chemistry. Advisor: Dr. Anne Hinderliter. 1 computer file (PDF): x, 127 pages.

Related to

Replaces

License

Series/Report Number

Funding information

Isbn identifier

Doi identifier

Previously Published Citation

Other identifiers

Suggested citation

Mahling, Ryan W. (2015). Communication in Membrane Repair. Retrieved from the University Digital Conservancy, https://hdl.handle.net/11299/180393.

Content distributed via the University Digital Conservancy may be subject to additional license and use restrictions applied by the depositor. By using these files, users agree to the Terms of Use. Materials in the UDC may contain content that is disturbing and/or harmful. For more information, please see our statement on harmful content in digital repositories.