Effects of Freezing and Freeze-drying on Protein Conjugated Nanoparticles
2018-10
Loading...
View/Download File
Persistent link to this item
Statistics
View StatisticsJournal Title
Journal ISSN
Volume Title
Title
Effects of Freezing and Freeze-drying on Protein Conjugated Nanoparticles
Authors
Published Date
2018-10
Publisher
Type
Thesis or Dissertation
Abstract
Biodegradable poly(D,L-lactide-co-glycolide) PLGA nanoparticles (NPs) have been extensively investigated for drug delivery. Protein conjugated NPs provide the advantage of active targeting. To overcome the instability associated with storage of aqueous dispersions, NPs are usually lyophilized. However, the freezing and freeze-drying stress can lead to nanoparticle aggregation and protein denaturation. As such, sucrose is widely used as a cryoprotectant and minimal ‘cryoprotectant to particle ratio’ is required. In this study, we used trypsin as a model protein to conjugate on the PEGlyated NPs. The effects of the freezing and freeze-drying process on trypsin conjugated NPs was studied by comparing the size of the NPs, the morphology of the NPs under fluorescent images and the trypsin activity after of the fresh made, freeze-thawed and lyophilized NPs. A minimum sucrose to NPs ratio was founded to provide the complete protection of the trypsin conjugated NPs.
Description
University of Minnesota M.S. thesis. October 2018. Major: Pharmaceutics. Advisors: Jayanth Panyam, Timothy Wiedmann. 1 computer file (PDF); viii, 58 pages.
Related to
Replaces
License
Series/Report Number
Funding information
Isbn identifier
Doi identifier
Previously Published Citation
Suggested citation
Su, Yafan. (2018). Effects of Freezing and Freeze-drying on Protein Conjugated Nanoparticles. Retrieved from the University Digital Conservancy, https://hdl.handle.net/11299/209180.
Content distributed via the University Digital Conservancy may be subject to additional license and use restrictions applied by the depositor. By using these files, users agree to the Terms of Use. Materials in the UDC may contain content that is disturbing and/or harmful. For more information, please see our statement on harmful content in digital repositories.