Evaluating use of AAV in reprogramming glial cells into neurons post stroke
2018-01
Loading...
View/Download File
Persistent link to this item
Statistics
View StatisticsJournal Title
Journal ISSN
Volume Title
Title
Evaluating use of AAV in reprogramming glial cells into neurons post stroke
Alternative title
Authors
Published Date
2018-01
Publisher
Type
Thesis or Dissertation
Abstract
Stroke is a leading cause of death, dementia, and long-term disability in the United States. Stroke is characterized by a loss of blood flow or damage to the central nervous system (CNS) and results in neuron death, reactive gliosis and inflammation. Early action is essential for stroke because every minute results in massive neuronal cell death yet 3-5% of patients receive care in time. Our goal is use an adeno-associated virus (AAV) to deliver Ascl1 or Ngn2 transcription factors that can induce reprogramming in reactive astrocyte to regenerate neurons in a mouse stroke model. Current AAV systems suffer from low conversion efficiency and off-target effects. Our approach is to study if glial specific promoters can increase conversion efficiency while limiting promiscuity.
Keywords
Description
University of Minnesota M.S. thesis.January 2018. Major: Stem Cell Biology. Advisor: Andrew Grande. 1 computer file (PDF); iii, 34 pages.
Related to
Replaces
License
Series/Report Number
Funding information
Isbn identifier
Doi identifier
Previously Published Citation
Other identifiers
Suggested citation
Dahlquist, Eric. (2018). Evaluating use of AAV in reprogramming glial cells into neurons post stroke. Retrieved from the University Digital Conservancy, https://hdl.handle.net/11299/194659.
Content distributed via the University Digital Conservancy may be subject to additional license and use restrictions applied by the depositor. By using these files, users agree to the Terms of Use. Materials in the UDC may contain content that is disturbing and/or harmful. For more information, please see our statement on harmful content in digital repositories.