Creation of Perfusable Tissue Engineering Constructs through Biological Assembly
2015-08
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Creation of Perfusable Tissue Engineering Constructs through Biological Assembly
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2015-08
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One of the biggest barriers in creating large tissues is the lack of oxygen and nutrient transport required for cell growth and tissue development in the interior region. The post-implantation cell survival in large tissue engineering constructs can be assisted by prevascularization. In this work we used a bottom-up approach to prepare large, prevascularized tissue constructs through perfusion culture of porous, cell-laden hydrogel constructs biologically assembled from smaller gel modules. The small gel modules had a controlled shape and were laden with HUVECs and hMSCs. They were packed in a bioreactor for perfusion culture, during which capillary formation inside and between individual gel modules led to the assembly of the small modules into a nearly centimeter sized porous construct. Viable cells and hollow lumen-like structures were observed throughout the porous construct, while in a nonporous control construct with similar dimensions viable cells were only observed in a peripheral layer several hundred micron thick. This modular assembly approach allows for creation of prevascularized large tissue constructs through the biological assembly of gels, which was difficult previously.
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University of Minnesota Ph.D. dissertation. 2015. Major: Chemical Engineering. Advisor: Wei Shen. 1 computer file (PDF); 102 pages.
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Riesberg, Jeremiah. (2015). Creation of Perfusable Tissue Engineering Constructs through Biological Assembly. Retrieved from the University Digital Conservancy, https://hdl.handle.net/11299/191459.
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