Between Dec 19, 2024 and Jan 2, 2025, datasets can be submitted to DRUM but will not be processed until after the break. Staff will not be available to answer email during this period, and will not be able to provide DOIs until after Jan 2. If you are in need of a DOI during this period, consider Dryad or OpenICPSR. Submission responses to the UDC may also be delayed during this time.
 

Acute contraction-induced strength loss, muscle inexcitability and recovery after exercise in males with Duchenne muscular dystrophy

Loading...
Thumbnail Image

Persistent link to this item

Statistics
View Statistics

Journal Title

Journal ISSN

Volume Title

Title

Acute contraction-induced strength loss, muscle inexcitability and recovery after exercise in males with Duchenne muscular dystrophy

Published Date

2020-11

Publisher

Type

Thesis or Dissertation

Abstract

This dissertation had three objectives with the overall goal to explore contraction-induced strength loss and recovery in males with Duchenne muscular dystrophy (DMD) specifically to: 1) evaluate a novel protocol, combining voluntary and evoked contractions to measure strength and excitability of wrist extensor muscles, for safety, feasibility, reliability and discriminant validity for males with DMD and aged-matched controls, 2) explore strength loss and muscle fiber inexcitability contribution to strength loss after submaximal isometric contractions in males with DMD, and. 3) determine the recovery of strength and muscle excitability immediately following contraction-induced force loss in males with DMD. Through the literature review, I discovered a need for a reliable and valid measurement protocol of muscle contractile function that could be used for DMD across all disease levels. Several measures for walking and upper extremity function are currently used to measure outcomes during clinical trials. However, none of the current measurements for DMD incorporate a way to quantitatively measure both voluntary and evoked strength along with muscle excitability over disease progression or in response to an intervention. The key to the assessment and protocol design needed to be feasibility and safety for boys and men with DMD at different stages of the disease process. I was able to design and evaluate a novel protocol, combining voluntary and evoked contractions to measure strength and excitability of wrist extensor muscles for safety, feasibility, reliability and discriminant validity between males with DMD and controls (Chapter 3). Wrist extensor muscle strength and excitability were assessed in males with DMD (N=10; mean 15.4 [SD 5.9] years) (Brooke 1-6) and age-matched healthy male controls (N=15; mean 15.5 [SD 5.0] years). Torque and EMG measurements were analyzed under maximum voluntary and stimulated conditions at two visits. I discovered that our protocol of multiple maximal voluntary contractions (MVC) and evoked twitch contractions was feasible and safe, with 96% of the participants able to complete the assessment protocol maintaining >93% strength both for DMD and controls (P≥0.074). Reliability was excellent for voluntary and evoked measurements. Torque, EMG and timing of twitch onset measurements discriminated between DMD and controls (P<0.001). This first part of the study demonstrated a useful protocol for measuring skeletal muscle function in clinical trials in males with DMD across various ages and disease levels. The second part of the study addressed in the context of a neuromuscular disease the concern of injury to dystrophic deficient skeletal muscle during and after repeated contractions (exercise) that result in acute strength loss. I knew that a feature of dystrophin-deficient skeletal muscle in the mdx mouse model was a hypersensitivity to strength loss from eccentric exercise due to fibers becoming unexcitable. I wanted to explore how this feature translated to humans lacking dystrophin. We hypothesized that there would be no difference in strength loss during exercise and that males with Duchenne muscular dystrophy (DMD) would have more significant impairment in muscle excitability corresponding with a loss of strength than age-matched controls (Chapter 4). Males with DMD and a group of age-matched controls performed a sub-maximal voluntary isometric wrist extensor exercise protocol until 55% of maximal voluntary contraction (MVC) could no longer be sustained. Voluntary and evoked force and EMG were accessed before, during, and after exercise. There was a significant interaction (time*group) (p<0.001) for MVC torque during exercise, suggesting that the two groups were different in how they reached muscle fatigue. No difference was measured between groups in MVC torque decrement at the time of exercise cessation (by design). Evoked twitch torque decrement was 34% for DMD and 36% for control with no group*time interaction observed (p=0.834). Muscle excitability contributed to the evoked torque variance in DMD (76%) and control (59%). The groups were not different in RMS EMG decrement (P=0.986) or M-wave decrement (P=0.911) during exercise, which does not support our hypothesis that the DMD groups had a more considerable decline in muscle excitability with fatigue during exercise. Lastly, the recovery of both strength and muscle excitability was explored and compared between groups. The DMD group recovered MVC baseline strength by 10 minutes post-exercise (P=0.530) and evoked torque by 5 minutes (P=0.266). In contrast, controls were still different from MVC and evoked torque baseline at 15 minutes after exercise (P<0.002). Strength loss from submaximal intermittent isometric exercise does not result in more significant impairment of muscle excitability is transient, and recovers faster in males with DMD than controls, suggesting a different mechanism of peripheral fatigue between the groups. The work contained in my dissertation begins to address the fear of exercise that has been a common concern for individuals with DMD and will contribute to knowledge of evidence-based exercise prescription in the future.

Description

University of Minnesota Ph.D. dissertation. November 2020. Major: Rehabilitation Science. Advisors: Dawn Lowe, Teresa Kimberley. 1 computer file (PDF); 145 pages.

Related to

Replaces

License

Collections

Series/Report Number

Funding information

Isbn identifier

Doi identifier

Previously Published Citation

Other identifiers

Suggested citation

Trost, Joyceann. (2020). Acute contraction-induced strength loss, muscle inexcitability and recovery after exercise in males with Duchenne muscular dystrophy. Retrieved from the University Digital Conservancy, https://hdl.handle.net/11299/218053.

Content distributed via the University Digital Conservancy may be subject to additional license and use restrictions applied by the depositor. By using these files, users agree to the Terms of Use. Materials in the UDC may contain content that is disturbing and/or harmful. For more information, please see our statement on harmful content in digital repositories.