Between Dec 19, 2024 and Jan 2, 2025, datasets can be submitted to DRUM but will not be processed until after the break. Staff will not be available to answer email during this period, and will not be able to provide DOIs until after Jan 2. If you are in need of a DOI during this period, consider Dryad or OpenICPSR. Submission responses to the UDC may also be delayed during this time.
 

Decellularized lung matrix as a scaffold for mouse lung stem cells

Loading...
Thumbnail Image

Persistent link to this item

Statistics
View Statistics

Journal Title

Journal ISSN

Volume Title

Title

Decellularized lung matrix as a scaffold for mouse lung stem cells

Published Date

2012-05

Publisher

Type

Thesis or Dissertation

Abstract

Decellularized tissue allows scientists to be able to examine cell proliferation and differentiation in the condition of a natural scaffold. The lung is one of several organs which were decellularized. This research proposes seeding decellularized lung with stem/progenitor cells. Endogenous lung stem cells were isolated by enzymatic digestion (Collagenase) of lung tissue and sorted based on expression of three markers: epithelial cell adhesion molecule EpCAM, (beta 4 integrin) CD104, and CD24. A cellular whole mouse lung matrix was prepared and complete decellularization shown by histology and PCR for genomic DNA. We expected the cells to survive and differentiate in this natural scaffold. However, the number of these cells was too low and cell survival was poor with no expression of any epithelial cell marker. The evidence in the literature shows that lung stem cells do exist but more work is required to investigate these cells cultured on a decellularized matrix.

Description

University of Minnesota M.S. thesis. May 2012. Major: Cell biology. Advisor: Dr. Angela-Panoskaltsis-Mortari. 1 computer file (PDF);vii, 48 pages.

Related to

Replaces

License

Series/Report Number

Funding information

Isbn identifier

Doi identifier

Previously Published Citation

Other identifiers

Suggested citation

Jaberansari, Ziba. (2012). Decellularized lung matrix as a scaffold for mouse lung stem cells. Retrieved from the University Digital Conservancy, https://hdl.handle.net/11299/131047.

Content distributed via the University Digital Conservancy may be subject to additional license and use restrictions applied by the depositor. By using these files, users agree to the Terms of Use. Materials in the UDC may contain content that is disturbing and/or harmful. For more information, please see our statement on harmful content in digital repositories.