Functions of NOM1 required for Cell Growth and Proliferation
2011-04-13
Loading...
View/Download File
Persistent link to this item
Statistics
View StatisticsJournal Title
Journal ISSN
Volume Title
Title
Functions of NOM1 required for Cell Growth and Proliferation
Authors
Published Date
2011-04-13
Publisher
Type
Presentation
Abstract
Our lab has identified a protein called NOM1 that is highly conserved from yeast to
humans and that we have shown is required for ribosome biogenesis. We have also
demonstrated that decreased levels of NOM1 result in cells that are smaller than
normal and that fail to proliferate. We hypthosize that NOM1 depletion activates a
p38 MAPK-dependent pathway that establishes both a G1 and a G2/M cell cycle
arrest. I am investigating the functions of NOM1 required for normal cell growth and
proliferation and am focusing on (1) NOM1’s ability to localize to and to target
proteins to the nucleolus, the sight of ribosome biogenesis, and (2) NOM1’s ability to
bind Protein Phosphatase I (PP1), a protein required for cell signaling, cell division,
and metabolism. This experiment is accomplished by first decreasing levels of
endogenous NOM1 in the cell using lentiviral vectors that encode short hairpin RNAs
that bind NOM1 mRNA and target it for degradation. Next, these cells are either left
untreated or transfected with one of three different NOM1 expression constructs. One
encodes the full length NOM1 protein that should rescue growth and proliferation of
the NOM1 depleted cells. The other two constructs contain either a deletion of the
nucleolar targeting domain of NOM1 or a mutation within the NOM1 PPI binding site.
The ability of these mutant constructs to rescue NOM1 depleted cells will reveal
whether either nucleolar targeting and/or PP1 binding is essential for NOM1 function.
These studies are important to define pathways and downstream events that respond
to deficiencies in ribosome production, a process disrupted in several human
diseases including several bone marrow failure syndromes.
Description
Additional contributor: Kathleen Conklin (faculty mentor)
Related to
Replaces
License
Series/Report Number
Funding information
Isbn identifier
Doi identifier
Previously Published Citation
Suggested citation
Meyer, Melissa. (2011). Functions of NOM1 required for Cell Growth and Proliferation. Retrieved from the University Digital Conservancy, https://hdl.handle.net/11299/104401.
Content distributed via the University Digital Conservancy may be subject to additional license and use restrictions applied by the depositor. By using these files, users agree to the Terms of Use. Materials in the UDC may contain content that is disturbing and/or harmful. For more information, please see our statement on harmful content in digital repositories.