Between Dec 19, 2024 and Jan 2, 2025, datasets can be submitted to DRUM but will not be processed until after the break. Staff will not be available to answer email during this period, and will not be able to provide DOIs until after Jan 2. If you are in need of a DOI during this period, consider Dryad or OpenICPSR. Submission responses to the UDC may also be delayed during this time.
 

A Multi-functional st-ELR Scaffold for Dentin Regeneration

Loading...
Thumbnail Image

Persistent link to this item

Statistics
View Statistics

Journal Title

Journal ISSN

Volume Title

Title

A Multi-functional st-ELR Scaffold for Dentin Regeneration

Published Date

2018-12

Publisher

Type

Thesis or Dissertation

Abstract

Pulpitis is one of the most widespread diseases in the world. Current advances in dental tissue engineering have provided an interesting alternative therapeutic approach in the field of regenerative endodontics. However, there remains a strong need to develop an optimized scaffold for supporting dentin regeneration. The objective of this PhD project is to develop a dental scaffold using elastin-like recombinamers(ELRs) to stimulate dentin regeneration while exhibiting antimicrobial ability to control potential re-infection of the pulp cavity. To provide a biomimetic scaffold that resembles the extracellular matrix in dentin tissue, we fabricated fibrous scaffold of ELRs using electrospinning technique and analyzed its ability in inducing biomimetic mineralization using the polymer-induced liquid precursor (PILP) process. The ELR scaffolds exhibited intra- and extra-fibrous mineralization, which highly mimicked the structure of mineralized native collagen in dentin. The scaffold is expected to be applied in the pulp cavity with direct contact with the pulp tissue. Therefore, we investigated the interaction between the mineralized ELR scaffold that contains statherin-derived peptide (st-ELR) and human dental pulp stem cells (hDPSCs). Proliferation and odontogenic differentiation of hDPSCs were analyzed and the study indicated that biomimetically mineralized st-ELR scaffold supported the proliferation and odontogenic differentiation of hDPSCs. Bacterial infection is considered as the major reason for the failure of implanted materials. Therefore, we functionalized st-ELR scaffold with antimicrobial peptides to prevent the potential infection caused by oral bacteria. A cysteine modified antimicrobial peptide GL13K(Cys-GL13K) was used in this study to achieve site-specific modification on the developed scaffold. First, we tethered Cys-GL13K peptides on titanium surface to analyze the properties and antimicrobial ability of immobilized peptides. A homogenous and strong coating of peptides was obtained. The tethered peptides exhibited promising antimicrobial ability against S. mutans, S. gordonii and E. faecalis. Furthermore, we bio-conjugated the peptides to st-ELR membranes using the same modification technique. Successful peptide modification was achieved, and the peptide functionalized st-ELR membrane exerted antimicrobial ability against S. mutans and S. gordonii. This research sheds light on the development and functionalization of scaffolds for the application of regenerating hard tissues such as dentin and bone. It allows the scaffold to highly resemble the architecture and physical properties of extracellular matrix in mineralized tissues. In addition, this research provides a new approach to modify the scaffold with diverse bioactive molecules to obtain multiple functions, while maintaining good interaction with native tissues.

Description

University of Minnesota Ph.D. dissertation. 2018. Major: Oral Biology. Advisor: Conrado Aparicio. 1 computer file (PDF); 163 pages.

Related to

Replaces

License

Collections

Series/Report Number

Funding information

Isbn identifier

Doi identifier

Previously Published Citation

Other identifiers

Suggested citation

LAN, CAIXIA. (2018). A Multi-functional st-ELR Scaffold for Dentin Regeneration. Retrieved from the University Digital Conservancy, https://hdl.handle.net/11299/218703.

Content distributed via the University Digital Conservancy may be subject to additional license and use restrictions applied by the depositor. By using these files, users agree to the Terms of Use. Materials in the UDC may contain content that is disturbing and/or harmful. For more information, please see our statement on harmful content in digital repositories.