Comparative Efficacy Study of IL-15 Transfections in Various Mouse Cancer Cells (RM-1 Prostate Cancer, B16 Melanoma, and 4T1 Breast Cancer)
2009-04-08
Loading...
View/Download File
Persistent link to this item
Statistics
View StatisticsJournal Title
Journal ISSN
Volume Title
Title
Comparative Efficacy Study of IL-15 Transfections in Various Mouse Cancer Cells (RM-1 Prostate Cancer, B16 Melanoma, and 4T1 Breast Cancer)
Alternative title
Authors
Published Date
2009-04-08
Publisher
Type
Presentation
Abstract
Genes for IL-15 were transfected into RM-1 (prostate cancer), B16 (melonoma), and 4T1 (breast cancer) cancer cells. The transfected genes consisted of two types, one for the isoform of IL-15 which has a full length leader sequence and is secreted from the cell, and the second for the isoform of IL-15 which has a truncated leader sequence and accumulates in the cytoplasm of the cell. Both kinds of each cancer cell line were cloned, showing that there was little translation of the IL-15 genes in RM-1 and 4T1 cells, and good translation of the IL-15 genes in B16. The vaccine efficacy of the original three cancer cell lines reflected these levels of IL-15. A genetic modification of the IL-15 gene was made to remove the signal peptide of the truncated isoform to make a very short sequence peptide (VSSP-1). The translation of the new genetically modified IL-15 gene was determined by cloning it into all of the cell lines, where it was found to give greater translation of IL-15. The vaccine efficacy for the various IL-15 transfectants of B16 melanoma was determined in mouse tumor studies. The VSSP-1 transfected B16 melanoma vaccine was the most efficacious.
Description
Additional contributor: W. Robert Fleischmann, Jr. (faculty mentor).
Related to
Replaces
License
Series/Report Number
Funding information
Isbn identifier
Doi identifier
Previously Published Citation
Other identifiers
Suggested citation
Schwab, Molly. (2009). Comparative Efficacy Study of IL-15 Transfections in Various Mouse Cancer Cells (RM-1 Prostate Cancer, B16 Melanoma, and 4T1 Breast Cancer). Retrieved from the University Digital Conservancy, https://hdl.handle.net/11299/50344.
Content distributed via the University Digital Conservancy may be subject to additional license and use restrictions applied by the depositor. By using these files, users agree to the Terms of Use. Materials in the UDC may contain content that is disturbing and/or harmful. For more information, please see our statement on harmful content in digital repositories.