Modeling Normal and Malignant Hematopoiesis Using Human Pluripotent Stem Cells
2011-01
Loading...
View/Download File
Persistent link to this item
Statistics
View StatisticsJournal Title
Journal ISSN
Volume Title
Title
Modeling Normal and Malignant Hematopoiesis Using Human Pluripotent Stem Cells
Authors
Published Date
2011-01
Publisher
Type
Thesis or Dissertation
Abstract
This thesis outlines progress made in creating several human pluripotent stem cell based models for studying hematopoiesis. Induced pluripotent stem cells were generated from human umbilical cord blood cells selected for the early hematopoietic progenitor marker CD34. Furthermore, reprogramming was attempted on primary leukemia samples to generate an induced pluripotent stem cell line with a genetic background harboring the mutations necessary for malignant hematopoiesis. Strategies for manipulating human embryonic stem cells to either express the bcr-abl oncogene or be deficient in the putative tumor suppressor gene sh2b3 were also developed, both genes of which are linked to the expansion of early hematopoietic progenitors.
Keywords
Description
University of Minnesota Master of Science thesis. January 2011. Major: Stem Cell Biology. Advisor: Dan S. Kaufman. 1 computer file (PDF); iv, 46 pages.
Related to
Replaces
License
Series/Report Number
Funding information
Isbn identifier
Doi identifier
Previously Published Citation
Suggested citation
Walsh, Patrick Joseph. (2011). Modeling Normal and Malignant Hematopoiesis Using Human Pluripotent Stem Cells. Retrieved from the University Digital Conservancy, https://hdl.handle.net/11299/104296.
Content distributed via the University Digital Conservancy may be subject to additional license and use restrictions applied by the depositor. By using these files, users agree to the Terms of Use. Materials in the UDC may contain content that is disturbing and/or harmful. For more information, please see our statement on harmful content in digital repositories.