Immobilized Laminin 332 And Ameloblastin Derived Peptides Increase Keratinocyte Adhesion On Titanium Substrates

Abstract

Laminin 332 participates in the formation of hemidesmosomes while ameloblastin is reportedly involved in the events following disruption of the periodontal ligament. Laminin 332 derived peptide (LAM), ameloblastin derived peptide (AMBN) or combinations of both were covalently immobilized on Ti discs. Immobilization of peptides was confirmed by contact angle, XPS, and fluorescent labelling. TERT-2 cells were cultured on LAM, AMBN or combinations of both peptides. Hemidesmosome formation and cell proliferation was assessed at 1, 24 and 48h. Statistical analysis with linear regression models was utilized (a=0.05) High amounts of well-anchored and homogeneously distributed peptides were identified on the Ti substrates. The immobilized peptides exhibited mechanical and thermochemical stability. TERT-2 cell proliferation was increased in the LAM or AMBN coated discs at 48h (p<0.05) while the combinations of both peptides induced the highest hemidesmosome formation at 48h (p<0.05).