Developing an Anti-Tumor Therapy: Expression of mLIGHT Cytokine in Escherichia coli & Salmonella enterica Typhimurium
2012-04-18
Loading...
View/Download File
Persistent link to this item
Statistics
View StatisticsJournal Title
Journal ISSN
Volume Title
Title
Developing an Anti-Tumor Therapy: Expression of mLIGHT Cytokine in Escherichia coli & Salmonella enterica Typhimurium
Alternative title
Authors
Published Date
2012-04-18
Publisher
Type
Presentation
Abstract
Current conventional cancer treatments such as chemotherapy and radiation have many limitations and adverse side effects. Salmonella enterica Typhimurium has been shown to colonize solid tumors, making this bacterium a potentially useful vector for delivering cytokines that promote tumor rejection. The objective of this project is to express mouse LIGHT (mLIGHT) cytokine, a tumor necrosis factor that promotes apoptosis, as a secreted protein in first E. coli and then S. enterica. Like S. enterica, E. coli is a Gram-negative facultative anaerobe, but can be used in a Biosafety Level 1 labtoratory. Soluble proteins, insoluble proteins, and secreted proteins were isolated from E. coli cultures harboring expression vectors that contain mLIGHT cDNA and the hemolysin secretion signal sequence under the control
of the Trc promoter. Western blotting indicated that mLIGHT is expressed as an insoluble protein, but not as a soluble or secreted protein. These findings suggest that other promoters and secretion signal sequences need to be tested to express mLIGHT as a secreted protein. Once a construct is made that results in expression of mLIGHT as a secreted protein in E. coli, the construct will be introduced into S. enterica. The bacterium will eventually be evaluated for its effectiveness as an anti-tumor therapy in mouse models of solid tumors.
Description
Faculty adviser: Dr. Janet Schottel
Related to
Replaces
License
Series/Report Number
Funding information
This research was supported by the Undergraduate Research Opportunities Program (UROP).
Isbn identifier
Doi identifier
Previously Published Citation
Other identifiers
Suggested citation
Ton, Angie. (2012). Developing an Anti-Tumor Therapy: Expression of mLIGHT Cytokine in Escherichia coli & Salmonella enterica Typhimurium. Retrieved from the University Digital Conservancy, https://hdl.handle.net/11299/123419.
Content distributed via the University Digital Conservancy may be subject to additional license and use restrictions applied by the depositor. By using these files, users agree to the Terms of Use. Materials in the UDC may contain content that is disturbing and/or harmful. For more information, please see our statement on harmful content in digital repositories.