Characterization of the Role of Macoilin in Dugesia japonica
2016-01
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Characterization of the Role of Macoilin in Dugesia japonica
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2016-01
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Praziquantel, the anti-schistosomiasis drug, is known to induce the bipolar (2-head) phenotypes in regenerative planarian flatworm Dugesia japonica, yet actual mechanism behind this head-instead-of-tail phenotype remains unclear. Previous studies has shown a specific voltage-gated calcium channel subtype, Cav1B, potentiates the bipolar phenotypes among regenerative planarians induced by praziquantel. Here I identify a new transmembrane protein, macoilin, in planarian that has the identical effects to that of Cav1B by RNAi methods. I have shown that by inhibiting the mRNA of macoilin, the regenerative worm under the exposure of praziquantel has a significant increase of bipolar phenotype penetrance compared to the naïve worms. Experimental data has also shown that the macoilin RNAi worms acquire defects in motility after regeneration. These results indicate a possible protein (macoilin) involved in praziquantel-induced signaling pathway that may bring light to the future studies of the mechanism of praziquantel.
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University of Minnesota M.S. thesis. January 2016. Major: Pharmacology. Advisor: Jonathan Marchant. 1 computer file (PDF); vi, 35 pages.
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Ren, Ruodi. (2016). Characterization of the Role of Macoilin in Dugesia japonica. Retrieved from the University Digital Conservancy, https://hdl.handle.net/11299/178919.
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