Effect of Endodontic Irrigation Protocols on Dentin Wettability and Tubule Penetration of Calcium Silicate Sealer

Abstract

Introduction: The effect of surfactants and ethanol on the wettability and tubule penetration of dentin by EndoSequence BC Sealer™ is investigated. Methods: Eighty human roots were instrumented and randomly divided into four groups (n=20) according to irrigation regimen: (1) no treatment, (2) SmearClear™, (3) 5.25% NaOCl/2% tween 80, (4) 5.25% NaOCl, 95% ethanol. Tubule penetration was evaluated via confocal laser scanning microscopy. Forty additional root sections were divided into four groups (n=10) and subjected to the irrigation regimens described above. Wettability was evaluated by contact angle measurement. Results: All tested solutions significantly reduced water contact angles, compared to control. Significantly greater mean and maximum tubule penetration was observed in the 5.25% NaOCl/tween 80 group, compared to the control. Conclusions: SmearClear™,17% EDTA/2% tween 80, and 95% ethanol significantly enhance wettability of human root dentin. Application of 17% EDTA/2%tween 80 prior to obturation significantly enhances tubule penetration of EndoSequence BC Sealer™.