The location, abundance, and proliferation status of SIV-specific CD8 cells in rhesus macaques vaccinated with live attenuated SIVΔnef and challenged with the pathogenic SIVmac251
2011-04-13
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The location, abundance, and proliferation status of SIV-specific CD8 cells in rhesus macaques vaccinated with live attenuated SIVΔnef and challenged with the pathogenic SIVmac251
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2011-04-13
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Abstract
Over 30 million people are infected with HIV/AIDS today. There is an
urgent need to develop an effective vaccine. Simian immunodeficiency
viruses (SIVs) in rhesus macaques serve as a valuable animal model of
HIV infection. Live attenuated SIVs provide protection in SIV infected
rhesus macaques challenged with highly pathogenic SIV. The goal of
this experiment is to identify correlations of protection that is provided by
SIVΔnef vaccination. Here I determined the abundance, location and
activation/proliferation status of SIV-specific CD8 T-cells in lymph nodes
and spleen from SIVΔnef vaccinated rhesus macaques before and after
challenge with the pathogenic SIVmac251. Confocal images were
obtained from tissues stained with MHC-tetramers that stain SIVspecific
CD8 T cells and Ki67 antibodies that stain proliferating cells and
activated T cells. We found similar numbers of SIV-specific CD8 T
cells, and similar percentages of SIV-specific CD8 T cells before and
after challenge. These results demonstrate that 1) SIV-specific CD8 T
cells were present in lymphoid tissues at the time of challenge, and 2)
no expansion of SIV-specific CD8 T cells in lymph nodes and spleen
was required for protection. This study yields insights into CD8 T cell
responses that are likely needed to be induced by a successful HIV
vaccine.
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Additional contributors: Arun Sasikala-Appukuttan; Ashley Haase; Paul Johnson; Hyeon Kim; Jung Joo Hong; Pamela Skinner (faculty mentor)
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Nguyen, Tammy Tran. (2011). The location, abundance, and proliferation status of SIV-specific CD8 cells in rhesus macaques vaccinated with live attenuated SIVΔnef and challenged with the pathogenic SIVmac251. Retrieved from the University Digital Conservancy, https://hdl.handle.net/11299/104734.
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