Expression of Capn6 During Osteoclast Differentiation In Wild-Type and Hdac4 Knockout Mice

Abstract

Introduction: The process of osteoclast differentiation consists of a network of complex signaling pathways. Previous studies suggest HDAC proteins play a suppressive role in osteoclast differentiation; however, not much is known about the specific role of HDAC4. Capn6 has been linked to increased organization of osteoclast microtubules for bone resorption. In this study, we observe the expression of Capn6 in wild-type and HDAC4 knockout osteoclasts. Methods: qRT-PCR was preformed to assess Capn6 expression in wild-type and HDAC4 knockout mice over days 0-4 of osteoclast differentiation. Results: Levels of Capn6 expression increased later in osteoclast differentiation in the wild-type osteoclasts, though the results were not significant. There was a significant increase in Capn6 in osteoclasts from HDAC4 knockout mice after 3 days of RANKL stimulation. This was also significant when comparing HDAC4 knockout to wild-type osteoclasts. Conclusion: HDAC4 may be a negative regulator of osteoclast function, suppressing the expression of Capn6.