Characterizing the Catalytic Activity and Specificity of RNF168 using Targeted Mass Spectrometry and Ubiquitination Stoichiometry-Based Quantitative Analysis
2021
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Characterizing the Catalytic Activity and Specificity of RNF168 using Targeted Mass Spectrometry and Ubiquitination Stoichiometry-Based Quantitative Analysis
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2021
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The Chen lab developed a new technique that uses quantitative proteomics approach for site-specific ubiquitination stoichiometry analysis, named the Isotopically Balanced Quantification of Ubiquitination (IBAQ-Ub), which is a method that quantifies the absolute abundance of ubiquitination. IBAQ-Ub and in vitro ubiquitination enzymatic assays were conducted to characterize the site-specific ubiquitination dynamics and linkage specificity of RNF168 in DDR(DNA-damage-response).
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Faculty advisor: Dr. Yue Chen
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This research was supported by the Undergraduate Research Opportunities Program (UROP).
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Wei, Lai. (2021). Characterizing the Catalytic Activity and Specificity of RNF168 using Targeted Mass Spectrometry and Ubiquitination Stoichiometry-Based Quantitative Analysis. Retrieved from the University Digital Conservancy, https://hdl.handle.net/11299/219533.
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