Derivation of lymphocytes from human induced pluripotent stem cells
2014-09
Loading...
View/Download File
Persistent link to this item
Statistics
View StatisticsJournal Title
Journal ISSN
Volume Title
Title
Derivation of lymphocytes from human induced pluripotent stem cells
Authors
Published Date
2014-09
Publisher
Type
Thesis or Dissertation
Abstract
Human pluripotent stem cells have the potential to produce essentially unlimited numbers of mature and functional blood lineage populations to study human hematopoiesis. Particularly, human induced pluripotent stem cells (hiPSCs) have the advantage to provide a source of autologous transplantable blood cell populations suitable for treatment of patient specific hematological diseases. This research aims to derive human lymphocytes from hiPSCs. There are three projects: The overall generation of human lymphocytes (B cell, T cell and NK cell) from hiPSCs is explored in Project I. In Project II and III, based on the derivation of NK cells, two human immunodeficiency disease models, both caused by specific somatic gene mutation, are established using human pluripotent stem cells. The ultimate goal of this research is to use hiPSCs to study the normal development of human lymphocytes in vitro, as well as model human immunodeficiency diseases by combining with gene therapy methods, thus providing a novel approach for immunotherapy. The hypothesis is that human lymphocytes can be derived from hiPSCs and this will enable the establishment of in vitro models to study human immunodeficiency diseases. Specific aims:1. To generate human lymphocytes from hiPSCs in vitro;2. To establish two human immunodeficiency disease models (X-SCID and WAS) through in vitro derivation of lymphocytes from hESCs/hiPSCs.
Description
University of Minnesota Master of Science thesis. September 2014. Major: Comparative and Molecular Biosciences. Advisor: Dan S. Kaufman. 1 computer file (PDF); v, 82 pages.
Related to
Replaces
License
Series/Report Number
Funding information
Isbn identifier
Doi identifier
Previously Published Citation
Suggested citation
Ma, Chao. (2014). Derivation of lymphocytes from human induced pluripotent stem cells. Retrieved from the University Digital Conservancy, https://hdl.handle.net/11299/167296.
Content distributed via the University Digital Conservancy may be subject to additional license and use restrictions applied by the depositor. By using these files, users agree to the Terms of Use. Materials in the UDC may contain content that is disturbing and/or harmful. For more information, please see our statement on harmful content in digital repositories.