Crystallization study of the resistance to cobalt and nickel repressor (RcnR) protein in complex with double-strand DNA

Li, Chao
2019-12

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http://hdl.handle.net/11299/211776
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Crystallization study of the resistance to cobalt and nickel repressor (RcnR) protein in complex with double-strand DNA

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2019-12

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This thesis included my work on the crystallization, data analysis and phasing attempts regarding RcnR in complex with a ds-DNA molecule .RcnR is a transcription factor that regulates the homeostasis of cobalt and nickel in bacterial cells. We crystallized Escherichia coli RcnR with DNA that encompasses the DNA binding site. X-ray diffraction data were collected to 2.9 Å. The crystal belongs to space group P61/522, with unit cell parameters a = b = 73.65 Å, c= 153.77Å, α=β=90°, γ = 120°. The second and third part included my work on MauG and PqqB projects.

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University of Minnesota Ph.D. dissertation. December 2019. Major: Biochemistry, Molecular Bio, and Biophysics. Advisor: Carrie Wilmot. 1 computer file (PDF); viii, 123 pages.

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