Identification of markers associated with race-specific resistance to Aphanomyces root rot in alfalfa, Poster at the 2017 APS meeting
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Identification of markers associated with race-specific resistance to Aphanomyces root rot in alfalfa, Poster at the 2017 APS meeting
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2017
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Abstract
Aphanomyces root rot, caused by Aphanomyces euteiches, is one of the most important diseases of
alfalfa in the United States. Two races of the pathogen are recognized and although most
cultivars are resistant to race 1, fewer have resistance to race 2, the predominant race in
North America. Molecular markers are needed to facilitate breeding for resistance and to
clarify race/resistance gene structure. Resistant and susceptible seedlings were identified
from three resistant cultivars, WAPH1, WAPH5 and 53V52, and used as parents to produce F1
populations. Severity of symptoms corresponded with amount of pathogen DNA and oospore density
in roots. Race-specific resistance involves a hypersensitive response of individual epidermal
or cortical cells upon pathogen attack followed by suberization of cells surrounding the stele
and strong autofluorescence in cortical cells, indicating the presence of phenolic compounds.
Segregation ratios of F1 populations suggested that resistance to race 1 in WAPH1 is
conditioned by a single gene but resistance to race 1 is multigenic in WAPH5 and 53V52, and
resistance to race 2 is multigenic in all three cultivars. Segregation for resistance to seven
strains of A. euteiches in 70 F1 full-sib plants derived from 53V52 suggested the presence of
clustered resistance genes and multiple race types. Identification of resistance gene loci is
in progress using genotyping by sequencing and genetic mapping of F1 populations.
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Samac, Deborah; Bucciarelli, Bruna; Dornbusch, Melinda; Miller, Susan; Yu, Long-Xi. (2017). Identification of markers associated with race-specific resistance to Aphanomyces root rot in alfalfa, Poster at the 2017 APS meeting. Retrieved from the University Digital Conservancy, https://hdl.handle.net/11299/194186.
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