Browsing by Subject "wheat leaf rust"
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Item Development of a genetic linkage map for Sharon goatgrass (Aegilops sharonensis) and mapping of a leaf rust resistance gene(Genome, 2013) Steffenson, Brian; Olivera, P.D.; Kilian, A.; Wenzl, P.Aegilops sharonensis (Sharon goatgrass), a diploid wheat relative, is known to be a rich source of disease resistance genes for wheat improvement. To facilitate the transfer of these genes into wheat, information on their chromosomal location is important. A genetic linkage map of Ae. sharonensis was constructed based on 179 F2 plants derived from a cross between accessions resistant (1644) and susceptible (1193) to wheat leaf rust. The linkage map was based on 389 markers (377 Diversity Arrays Technology (DArT) and 12 simple sequence repeat (SSR) loci) and was comprised of 10 linkage groups, ranging from 2.3 to 124.6 cM. The total genetic length of the map was 818.0 cM, with an average interval distance between markers of 3.63 cM. Based on the chromosomal location of 115 markers previously mapped in wheat, the four linkage groups of A, B, C, and E were assigned to Ae. sharonensis (Ssh) and homoeologous wheat chromosomes 6, 1, 3, and 2. The single dominant gene (designated LrAeSh1644) conferring resistance to leaf rust race THBJ in accession 1644 was positioned on linkage group A (chromosome 6Ssh) and was flanked by DArT markers wpt-9881 (at 1.9 cM distal from the gene) and wpt-6925 (4.5 cM proximal). This study clearly demonstrates the utility of DArT for genotyping uncharacterized species and tagging resistance genes where pertinent genomic information is lacking.Item SNP data of 556 isolates of the wheat leaf rust fungus, Puccinia triticina from 11 world-wide regions(2019-11-07) Kolmer, James; Herman, Adam; Ordonez, Maria; German, Silvia; Morgounov, Alexy; Pretorius, Zack; Visser, Botma; Anikster, Yehoshoa; Acevedo, Maricelis; JKolmer@umn.edu; Kolmer, James; USDA-ARS Wheat Leaf rust research project | USDA ARS Cereal Disease LabThis dataset includes SNPs of 556 isolates of the wheat leaf rust fungus, Puccina triticina from 11 worldwide regions that were mapped to the reference genome. This data was used to examine the population structure of Puccinia triticina on a worldwide basis, and to examine the evolutionary relationship between collections from a wheat progenitor, collections from durum wheat, and collections from common wheat. Groups of isolates that were highly related were found in distant continental regions, indicating past and current migration of this important wheat pathogen. Data is being released now in conjunction with publication of a primary research paper that describes this research.