Browsing by Subject "viability"
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Item Complete Data for: Desiccation tolerance of the invasive alga starry stonewort (Nitellopsis obtusa) as an indicator of overland spread risk(2021-10-13) Glisson, Wesley J.; Wagner, Carli K.; Verhoeven, Michael R.; Muthukrishnan, Ranjan; Contreras-Rangel, Rafael; Larkin, Daniel J.; wjglisson@gmail.com; Glisson, Wesley, J; Minnesota Aquatic Invasive Species Research CenterThe ability of invasive macrophytes to survive out of water, i.e., their desiccation tolerance, is an important indicator of capacity for spread to new waterbodies through overland transport. Invasion by the alga starry stonewort (Nitellopsis obtusa [Desv. in Loisel.] J. Groves; Characeae) in North America is likely driven via overland transport, but little is known regarding its ability to remain viable out of water. We conducted laboratory and outdoor experiments to evaluate desiccation tolerance of N. obtusa propagules, including single stem fragments, small and large clumps of fragments, and bulbils (asexual reproductive structures). Propagules were removed from water after 15 min to 5 d to identify desiccation thresholds. The data from these experiments are documented and available here for public availability and use.Item Complete Data for: Laboratory evaluation of copper-based algaecides for control of the invasive macroalga starry stonewort (Nitellopsis obtusa)(2022-05-23) Glisson, Wesley J; Contreras-Rangel, Rafael; Bishop, West M; Larkin, Daniel J; wjglisson@gmail.com; Glisson, Wesley J; Minnesota Aquatic Invasive Species Research CenterNitellopsis obtusa ([Desvaux] J. Groves [1919]; Characeae), known as starry stonewort, is an invasive macroalga in Laurentian Great Lakes states and provinces in North America. Because of its potential negative impacts on native ecosystems and recreation, N. obtusa has become a high-priority target for management. However, there is a critical lack of foundational information on the efficacy of different algaecides, and concentrations thereof, for N. obtusa control. Additionally, control of N. obtusa bulbils—asexual reproductive structures that are the main pathway for the establishment of new plants—has proven difficult. We tested the efficacy of six commonly used copper-based algaecides, at a series of copper concentrations up to the maximum labeled rate, on N. obtusa thalli (photosynthetic aboveground tissues) and bulbils in controlled laboratory experiments. Bulbils were placed above and below sediment in separate experiments to evaluate whether sediment acted as a barrier to treatment. The data from these experiments are provided and documented here.Item Data for Impacts of Nanoplastics on the Viability and Riboflavin Secretion in the Model Bacteria Shewanella oneidensis(2020-06-30) Fringer, Victoria S; Fawcett, Liam P; Mitrano, Denise M; Maurer-Jones, Melissa A; maujones@d.umn.edu; Maurer-Jones, Melissa ACharacterizing the impact of nanoplastics to organism health is important to understand the consequences of the environmental plastic waste problem. This article examines the impact of nano-polystyrene (nano-PS; 159±0.9 nm diameter) to ecologically relevant bacteria Shewanella oneidensis. Bacterial viability was evaluated using a growth-based assay. Riboflavin secretion is a critical cell function of S. oneidensis, serving as an electron mediator in anaerobic respiration and/or as a signaling molecule when the bacteria are under stress. Thus, changes in cellular function were monitored through riboflavin secretion in order to evaluate toxic responses that may not result in cell death. Under aerobic and anaerobic exposures (4, 8 or 12 h), the viability of the S. oneidensis was minimally changed as compared to the control, while the concentration of riboflavin secreted varied with exposure dose. In order to determine if this was a specific response to nanoplastic particles, opposed to a response to either particles or plastic more generally, we exposed the system to colloidal TiO2 nanoparticles and polystyrene and polyethylene thin films. We confirmed that riboflavin secretion trends were specific to nano-PS and not to these other materials, which showed no significant changes. We investigated the association of the nano-PS with ICP-MS using Pd that was chemically incorporated into the model nanoplastics. While 59.2% of the nano-PS were found in the non-cellular culture media, 7.0% and 6.6% was found associated with the loosely and tightly bound extracellular polymeric substance, respectively. There was significantly more nano-PS (10.9%) strongly associated with the cells. Taken together, we found that nano-PS had minimal impacts to viability but caused a significant change in the function of S. oneidensis that can be related to the nano-PS attached or in proximity to the bacterium. These trends are consistent between aerobic and anaerobic cultures, signifying that the stress response of S. oneidensis can be generalized between different environmental compartments. This work highlights that the association of nanoplastic materials with microorganisms may modify the cellular function that could ultimately be an impact to ecosystem health.Item Restorable Wetland Decision Support Data, 2014(2024-02-01) Johnson, Lucinda; Brady, Valerie; Erickson, Jeremy; Brown, Terry; Gernes, Mark; ljohnson@d.umn.edu; Johnson, Lucinda; Natural Resources Research InstituteThe Minnesota Restorable Wetland Decision Support Data were developed in combination with the Minnesota Restorable Wetland Index to: predict likely locations of restorable wetlands; locate highly stressed areas most in need of water quality or habitat improvement; prioritize areas that already are or are most likely to result in high functioning, sustainable wetlands; identify areas that will provide the greatest benefits in the form of water quality and habitat. Data include: Minnesota Restorable Wetland Decision Support - Viability, Minnesota Restorable Wetland Decision Support - Water Quality Benefits, Minnesota Restorable Wetland Decision Support - Habitat Stress, Minnesota Restorable Wetland Decision Support - Habitat Benefits, Minnesota Restorable Wetland Decision Support - Nitrogen Stress, and Minnesota Restorable Wetland Decision Support - Phosphorus Stress. This data had previously been available within the Minnesota Restorable Wetland Prioritization Tool (2013-2024).