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Browsing by Subject "silver nitrate"

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    The Effect of Blood Contamination on the Retrograde Sealing Ability of Biodentine: A Micro-CT Analysis
    (2019-08) Dimond, Colby
    AIMS To evaluate the sealing ability of Biodentine in its use as a root end filling material. To evaluate how the sealing ability of Biodentine would be affected by contamination with blood. To evaluate the use of micro-CT and silver nitrate as a method to examine sealing ability and the microleakage of different dental materials. MATERIALS AND METHODS: Thirty single rooted teeth were selected as sample teeth. Sample teeth were prepared and obturated using gutta-percha and Ah Plus sealer. The samples were coated with nail polish, resected 3mm apically with a 0 degree bevel angle. Samples were labeled, each was apically prepared 3mm using an ultrasonic tip. Samples were assigned at random to either the uncontaminated sample group or the blood contaminated sample group. All the samples were filled using Biodentine following manufacturer’s instructions. For the blood contaminated sample, one drop of blood was placed into each root end preparation. The blood was left in place for 1 minute and then air dried, leaving blood on walls of the retropreparation. The blood contaminated samples were filled with Biodentine. All of the prepared sample groups, were placed in PBS for one week to allow for proper setting of the Biodentine material. A 25% w/w solution of silver nitrate solution was used to soak the samples overnight and allow penetration into the samples. Samples were scanned using a micro-CT unit. For each component (silver nitrate, Biodentine, voids), an object volume was calculated using the CT-Analyzer software. An overall volume of the root end preparation was calculated to compare each component in order to get a percentage. The depth of the root end preparation and depth of silver nitrate penetration were calculated according to the voxel size and the number of slices included for each sample. The data were entered into an Excel spreadsheet and statistical analysis was completed using GraphPad Prism Version 8.1.2 statistical software. RESULTS: A significant difference was found between the two sample groups in the percentage of silver nitrate volume compared to the overall retropreparation volume, with the blood contaminated group showing less penetration of silver nitrate. A significant difference was found in the void percentage of the total volume between the two groups, with the blood contamination group showing more voids in the root end filling material. No statistical difference was noted between the depth of silver nitrate penetration between the two groups. CONCLUSIONS: Blood contamination does not affect the sealing ability of Biodentine, when it is used as a root end filling material. The presence of blood in the root end preparation significantly increases the volume of voids present in the root end filling material. The combination of silver nitrate as a tracer and micro-CT to evaluate microleakage is a promising study design for future applications
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    Effect Of Placing A Glass Ionomer Restoration Before Setting Of A Grey Mta Furcation Perforation Repair Using Micro-Computed Tomography
    (2017-08) Brown, Alexander
    Introduction The aims of the study were to determine if restoration with glass ionomer over unset MTA affects the amount of silver nitrate penetration in a furcation perforation repair and to evaluate micro-computed tomography as a technique by which to quantitatively measure marginal integrity in endodontic research. Methods Twenty-two previously extracted human mandibular molars were sectioned to isolate a furcation region section. Each sample had a furcation perforation induced using a bur. Samples were randomly divided into two experimental groups. Group 1 was restored with grey MTA followed immediately by a resin-modified glass ionomer, while group 2 had the placement of resin-modified glass ionomer delayed for approximately 72 hours. 2 samples were used as negative controls that would not be exposed to silver nitrate. Experimental group samples were submerged in a radiopaque silver nitrate solution (25% w/w) for six hours. All samples were then scanned using a micro-computed tomography machine. The volume of radiopacity above a threshold level was then used to determine the volume of silver nitrate penetration. Results Samples that were immediately covered with resin-modified glass ionomer (group 1) demonstrated less silver nitrate penetration than those that had been covered after full setting of the MTA. The mean volumes above the radiographic threshold for group 1 and group 2 were 0.24 ± 0.27 and 0.54 ± 0.25 mm3, respectively. The difference between the two groups was statistically significant (p < 0.05). Conclusions In a furcation perforation repair model, silver nitrate solution penetration is lower in grey MTA restorations that are immediately covered by glass ionomer on the pulp chamber floor side than those that were covered after 72 hours. Silver nitrate penetration and micro-computed tomography is a feasible option for the study of interface integrity in endodontics.