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Browsing by Subject "microsatellite"

Now showing 1 - 2 of 2
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    Evaluating the ability of microsatellite DNA markers and otolith microchemistry to distinguish spatially separated populations and identify recruitment sites of common carp (Cyprinus carpio) in interconnected lake systems of the North American Midwest
    (2017-10) Swanson, Reid
    The common carp (Cyprinus carpio) is an invasive fish whose populations have grown to ecologically damaging levels in the North American Midwest and many areas throughout the world. Recent research has shown that abundance of this species in areas of the North American Midwest is driven by its propensity to use shallow basins as productive nursery habitats. The ability of managers to discriminate which shallow basins are producing carp across a large sub-watershed of interconnected lakes has the potential to increase the efficacy of management practices which are aimed at the disruption of successful recruitment (i.e. surviving to join adult population). This study assessed whether carp nurseries could be distinguished based on differences in 12 microsatellite DNA markers in carp across the twin cities metropolitan area (n=1023) and the concentrations of 11 trace elements measured in carp otoliths collected in the Six-Mile Creek sub-watershed, Minnesota, USA (n=157). I found that genetic assessment could separate carp populations at a regional scale, but not between individual putative nurseries. Microchemical otolith analysis revealed that it is feasible to classify carp to their capture locations, discriminate between nursery and non-nursery habitat types, and distinguish juvenile carp from individual nursery sites. Elemental signatures of otoliths were obtained from laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). Ratios of aluminum (Al), barium (Ba), copper (Cu), iron (Fe), potassium (K), lithium (Li), magnesium (Mg), sodium (Na), phosphorus (P), and strontium (Sr) to calcium (Ca) in otolith edges differed significantly among carp from all eight capture sites in a 70 km2 watershed. Ratios of Ba, Fe, Li, manganese (Mn), and P to Ca differed significantly among juvenile carp from three nursery basins. Ratios of Al, Ba, Cu, Fe, Li, K, Na, P, and Sr to Ca were significantly different between nursery and non-nursery habitat types. Quadratic discriminant analysis (QDA) could accurately classify otoliths to collection site (total accuracy 54%). QDA had increased accuracy when restricted to juvenile carp (76%) and classifying carp to nursery and non-nursery habitat types (87%) in contrast to individual sites. Further evaluation of differences between elemental signatures of the core and edge region (i.e. recent and natal signatures) and water samples from multiple years suggests that elemental parameters in otoliths and water are changing across time preventing identification of past recruitment sources. Identification of past natal origins of carp in these systems using otolith microchemistry will require obtaining continuous signatures across larger spatial and temporal scales.
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    Sample and genetic data: Muskellunge (Esox masquinongy) movement patterns and habitat use in the St. Louis River Estuary and southwestern Lake Superior
    (2019-09-23) Schaeffer, Erin; Miller, Loren M; Pinkerton, Jeramy; Venturelli, Paul A; LMM@umn.edu; Miller, Loren M
    Sample data and microsatellite genotypes for 258 muskellunge, including 60 with acoustic tags, collected in 2017 from the St. Louis River estuary of Lake Superior. Genetic data were used to assign strain ancestry from two stocked sources (from Minnesota and Wisconsin). Ancestry, sex and length were used as variables when examining movement patterns using an acoustic receiver array in the estuary and Lake Superior.