Browsing by Subject "blood"
Now showing 1 - 4 of 4
Results Per Page
Sort Options
Item Blood Pressure(2008-04-07) Renner, JodiThis document provides a description of blood pressure, ranges of normal and high blood pressure, and recommendations on how lifestyle modifications can help decrease blood pressure. Finally, it describes some classes of common medications that may be used to help lower blood pressure.Item Dependence of Whole Blood Rheology on Oxygen Tension in Sickle Cell DIsease(2018-02) Lu, XinranIn sickle cell disease, oxygen tension plays a major role in dictating the mechanical function of single red blood cells. As oxygen tension decreases, cellular stiffness increases, to the point where the flow of whole blood can come to a compete occlusion. Unfortunately, there are also many unknowns in sickle cell disease, which is clinically expressed by the severe lack of approved treatment options for the disease. To solve many of these issues, we aim to expand our understanding of sickle cell disease by diving into the fundamental mechanisms by which the whole blood rheology becomes impaired in response to oxygen tension. Here, we use an in vitro disease model of sickle cell rheology, built within a microfluidic platform, to simulate whole blood flow within the microvasculature. We first report on the relationship between whole blood rheology and oxygen tension in sickle cell disease under steady state conditions and map out the specific oxygen tension where rheological impairment of blood flow begins. We then report on this measurement in both sickle cell trait (the heterozygous carrier state of sickle cell disease) as well as transfusion therapy in sickle cell disease, where we find and report on the rheological differences compared to native sickle cell disease. Next, we modify our microfluidic disease model to investigate the temporal and spatial dynamics of sickle cell disease by creating a microvascular capillary tree design couple to physiologically relevant oxygen tension gradients. Finally, we revisit the specific response of whole blood rheology as a function of oxygen tension by mapping out the velocity gradients and velocity profiles of blood flow, where we find characteristic differences in velocity profile shapes relative to oxygen.Item Differentially Expressed Gene Transcripts Using RNA Sequencing from the Blood of Immunosuppressed Kidney Allograft Recipients(2016-02-29) Dorr, Casey; Wu, Baolin; Guan, Weihua; Muthusamy, Amutha; Sanghavi, Kinjal; Schladt, David; Maltzman, Jonathan; Scherer, Steven; Brott, Marcia; Matas, Arthur; Jacobson, Pamala; Oetting, William; Israni, Ajay; isran001@umn.edu; Israni, AjayThis is the FPKM and clinical covariate data from a paper in PLOS One. These data will be useful for future researchers to study gene expression patterns over time before and after immunosuppression and kidney transplantation. We removed subject names and any other identifiers in order to de-identify the subjects.Item The Effects of Therapeutic Strategies in Restoring Sickle Cell Disease Blood Rheology(2022-04) Hansen, ScottSickle cell disease is a hereditary disease of the hemoglobin with devastating acute and chronic complications. The pathological polymerization of sickle hemoglobin during hypoxia reduces red blood cell deformability and increases blood viscosity. These biophysical changes to the red blood cells and whole blood rheology can obstruct blood flow and contribute to vaso-occlusion in the microcirculation. Though the genetic and molecular basis for the disease has been understood for decades, limited treatment options are available to those who suffer from this disease. Microfluidic platforms provide a physiologically relevant pre-clinical model to assess the response of sickle cell blood rheology to therapeutic strategies in vitro. This work focuses on the roles of affinity modifying compounds and high expression of fetal hemoglobin in inhibiting sickle hemoglobin polymerization and restoring healthy blood rheology. Isolating the biophysical effects of these therapeutic strategies on blood flow provides a better understanding of their mechanisms of action that may be of clinical significance. Microfluidic studies of sickle cell disease blood flow may help accelerate drug development and improve patient outcomes.