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Browsing by Subject "School of Medicine"

Now showing 1 - 3 of 3
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    A fast and efficient method to generate contracting embryoid bodies from murine iPs cells
    (2012-04-18) Chen, Denise
    This project aimed to test and refine a method of differentiating iPs cells into contracting embryoid bodies. Embryoid bodies (EBs) are spheroid clumps of pluripotent cells that can differentiate into various types of cells5. Spontaneous contraction of EBs indicates development into cardiomyocytes. The ability to differentiate cardiomyocytes from iPs cells can then aid in the process of cardiomyocyte regeneration for individuals who have suffered significant and damaging cardiac events. Our research aimed to develop a method of quickly and efficiently differentiating murine iPs cells into contracting EBs. Our protocol yielded areas of spontaneously contracting proportions of EBs within six days, and the peak proportion of contracting EBs was observed within nine to ten days. Further research into this topic may look into the role of norepinephrine in successfully developing EBs, or the applicability of this method of differentiation on human iPs cells.
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    Incontinence in Elite Female Athletes
    (2010-12-15) Less, Rebekah
    Urinary incontinence has been a common problem in female athletes. In previous studies it has been shown that over 50% of elite female athletes have experienced inadvertent urine loss1. There are three different types of urinary incontinence: stress, urge, and mixed2. The stress related incontinence is defined as the “involuntary leakage on effort or exertion”3, examples of such effort include, but are not limited to, sneezing, coughing, and exercise. The urge related incontinence is defined as the “involuntary leakage accompanied by or immediately proceeded by urgency.”3 Finally the mixed related incontinence is defined as the “involuntary leakage associated with urgency and also with exercise, effort, sneezing, and coughing.”3
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    Protein Synthesis Is Required for Recovery of Escherichia coli from Desiccation Stress
    (2009-04-08) Grunewald, Justin
    The stress of desiccation is fatal to many cells and can induce significant water loss, which can impact the structure and function of membranes. When put under the stress of desiccation, E. coli cells are thought to enter a Viable But Nonculturable state (VBNC). The VBNC state is brought on by various stresses and leads to absence of cell division, reduced activity, but cells remain viable. By adding chloramphenicol to cells, the importance of protein synthesis during desiccation and rehydration was investigated. Understanding the mechanism required for recovery from the VBNC state is important in determining ways to improve microbial inhabitants in clinical and agricultural settings.