Browsing by Subject "SEM"
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Item Fundamental tradeoffs generating the worldwide leaf economics spectrum(Ecological Society of America, 2006) Shipley, Bill; Lechowicz, Martin J; Wright, Ian; Reich, Peter BRecent work has identified a worldwide “economic” spectrum of correlated leaf traits that affects global patterns of nutrient cycling and primary productivity and that is used to calibrate vegetation–climate models. The correlation patterns are displayed by species from the arctic to the tropics and are largely independent of growth form or phylogeny. This generality suggests that unidentified fundamental constraints control the return of photosynthates on investments of nutrients and dry mass in leaves. Using novel graph theoretic methods and structural equation modeling, we show that the relationships among these variables can best be explained by assuming (1) a necessary trade-off between allocation to structural tissues versus liquid phase processes and (2) an evolutionary trade-off between leaf photosynthetic rates, construction costs, and leaf longevity.Item Molecular systematics and morphological congruence in the Pezizales and Neolectales (Ascomycota): three case studies(2013-07) Healy, Rosaria AnnA revolution in fungal systematics is underway due to the application of molecular phylogenetic analyses to previously intractable problems posed by unculturable fungi, unlinked lifecycle stages, and hidden diversity. Three chapters here detail how molecular phylogenetic analyses provided a taxonomic framework for early derived lineages of ascomycetes (Neolectales and Pezizales) upon which morphology could be re-examined for homology, linkage of lifecycle stages, and reconstruction of character states. Mitospores of fungi are rarely linked to meiospore stages in nature, unless they are temporally or spatially coordinated, or one form produces the other in culture. In the first chapter molecular phylogenetic analyses of ITS (a fungal barcode) and 28S rDNA are used to link mitotic sporemats that are produced on the soil surface with rarely cultured or unculturable ectomycorrhizal Pezizales. In this study, 48 OTUs representing six independent ectomycorrhizal lineages were delimited from 292 spore mats collected in Asia, Europe, South America, and North America. Most were truffle lineages, but one lineage included above ground cupuliform fungi, and one lineage had no detected meiospore stage. The discovery that a high diversity of mitospore-mat producing ectomycorrhizal Pezizales are common and widely distributed across the world implies that mitospores play an important role in the lifecycle of these organisms. Neolecta, the only fruit-body forming extant genus of the earliest derived lineage of ascomycetes, was previously determined to be phylogenetically related to non-ascoma forming Taphrinomycotina. The second chapter presents research on septal pore characters in Neolecta vitellina to investigate whether the ascoma in Neolecta is analogous or homologous to later derived lineages of ascoma forming Pezizomycotina. Two unique structures were associated with the septal pores in Neolecta: a vacuolar crystal that lodged within the septal pore of disrupted cells, and a membranous matrix that plugged the pores. The Neolecta crystal appears to be similar in function to the Woronin body of later derived lineages, but differs by the organelle in which it is formed, the numbers of crystals formed per organelle, and the association of vesicles with the crystal. Unlike Pezizomycete septal pore structures the membranous matrix of Neolecta septal pores is not confined to the septum. These two unique structures are presented as evidence of an independent evolution of the Neolecta ascoma. Truffles have evolved at least 16 times from cupuliform Pezizales. Previous phylogenetic analyses have inferred that reversals from truffles to cupuliform fruitbodies are unlikely. Chapter 3 details the phylogenetic analyses of multilocus alignments from world-wide collections of Pachyphloeus (truffles) and Scabropezia (cupuliform fungi). In these analyses, Scabropezia was inferred to be embedded within Pachyphloeus, and a truffle was reconstructed as the ancestral form of this lineage, perturbing the idea that cup fungi have not evolved from truffles. It is likely that taxa are missing from this study that could change these results. All described species in the lineage were transferred to Pachyphlodes to redress the illegitimacy of "Pachyphloeus." Eight lineages with 45 OTUs were delimited, expanding the diversity in this genus 3-fold. Spore wall development was useful for interpreting differences in spore ornamentation among the eight lineages.Item Multidimensional well-being of residents affected by the Pak Mun Dam, Thailand(SSBFNET, 2021-03) Chaiyamart, PattaraphongpanUnderstanding the effects on the overall well-being of the communities affected by the Pak Mun Dam construction would fill in the gaps in existing knowledge and facilitate a deeper discussion of the factors that impact overall wellbeing. The Pak Mun Dam was finished in 1994 and from that point on there have been changes affecting local residents. This study attempts to understand the impact of those changes from a well-being perspective using primary data collected from residents in villages close to the dam site. Structural Equation Model (SEM) was a method for this research analysis. Results from a structural model show that social and economic well-being are important factors that impact the overall well-being of the affected residents from the Pak Mun Dam area. Furthermore, we discuss how information from 20 items within seven dimensions of the well-being measurement model can be used to develop more efficient strategies and policies to increase well-being capital.