Browsing by Subject "RiPPs"

Now showing 1 - 2 of 2
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    Borosin foray: Expanding a family of fungal autocatalytic α-N-methylating RiPP natural products
    (2020-07) Quijano, Marissa
    Backbone N-methylations impart several favorable characteristics to peptides including increased proteolytic stability and membrane permeability. Nonetheless, amide bond N-methylations incorporated as post-translational modifications are scarce in nature and were first demonstrated in 2017 for a single set of fungal metabolites. Here we expand on our previous discovery of iterative, autocatalytic α-N-methylating precursor proteins in the borosin family of ribosomally encoded peptide natural products. We identify over 50 putative pathways in a variety of ascomycete and basidiomycete fungi and functionally validate nearly a dozen new self-α-N-methylating catalysts. Significant differences in precursor size, architecture, and core peptide properties subdivide this new peptide family into three discrete structural types. Lastly, using targeted genomics, we link the biosynthetic origins of the potent antineoplastic gymnopeptides to the borosin natural product family. This work highlights the metabolic potential of fungi for ribosomally synthesized peptide natural products.
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    Supplementary raw data for α‐N‐Methyltransferase regiospecificity is mediated by proximal, redundant enzyme–substrate interactions
    (2025-01-27) Crone, Kathryn K; Labonte, Jason W; Elias, Mikael H; Freeman, Michael F; mffreema@umn.edu; Freeman, Michael F; Freeman Lab University of Minnesota
    The data deposited here are raw mass spectrometry files associated with the results presented in the paper, "α‐N‐Methyltransferase regiospecificity is mediated by proximal, redundant enzyme–substrate interactions." These data have been made publicly available in keeping with our lab's data availability policy.