Browsing by Subject "Resorption"

Now showing 1 - 3 of 3
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    An In Vitro Study Of Ph Changes With Endosequence Bc Root Repair Material Fast Set Putty In Simulated Resorptive Defects
    (2018-08) Kickertz, Katie
    One of the challenges to endodontic treatment is external inflammatory root resorption, a destructive process which destroys the radicular portion of the tooth when under pathologic states. Treatment has traditionally been via long-term placement of an intracanal medicament of calcium hydroxide. Calcium hydroxide is antimicrobial, able to dissolve tissue, inhibit resorption and induce hard tissue formation. It has an alkaline pH, which disrupts bacterial cell wall metabolism. Cons to its use include potential for tooth fracture and multiple appointments needed for effect on the resorption process. The purpose of this study was to compare in vitro the effects of intracanal placement of Endosequence BC RRM Fast Set Putty and calcium hydroxide on hydroxol ion diffusion through dentin of teeth with simulated root surface cavities, mimicking external resorptive lesions. The goal was to determine if Endosequence BC RRM Fast Set Putty is a conceivable alternative to calcium hydroxide in cases of external inflammatory resorptive lesions.
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    pH Change Following Smear Layer Removal and Final Rinse with 95% Ethyl Alcohol Using Calcium Hydroxide Medicament or BC Sealer
    (2016-10) Saylor, Christopher
    Introduction: An in vitro investigation of pH change at the external root surface after final rinse of 95% ethyl alcohol using Ultracal XS, Calasept Plus, or obturation with BC sealer. Methods: 80 single rooted extracted human teeth were decoronated with a standardized root length of 15mm. All root were instrumented to size F4 using the ProTaper Gold file system. Defects were made on the facial and lingual root surfaces to remove cementum. Teeth were then randomized into treatment groups based on medicament used and final rinse with or without ethanol. Negative controls received no medicament. Full wax control groups received medicaments but were completely sealed with wax. After final rinses roots were filled with Ultracal XS, Calasept Plus, or BC sealer. Negative controls received no medicaments. Roots were sealed at coronal and apical ends using two layers of sticky wax and were placed in a 4mL lab vial containing 2mL of sterile saline. pH was measured days 0,1,2,4,7,9,12,16,22,28. Tooth roots were sectioned and remaining dentin thickness (RDT) was measured to correlate RDT and pH values. A multiple linear regression model was used to estimate the effects of medicament, ethanol, interaction of medicament and ethanol, as well as a continuous variable of RDT. Pairwise comparisons were completed to establish differences within groups. Results: It appears that ethanol may have an effect on the rate of pH change in the Calasept Plus group. Trends towards a faster rise in pH were noted in both ethanol positive calcium hydroxide groups. BC sealer maintained a pH higher than negative controls for the first 12 days before dropping to baseline levels. RDT was found to affect pH levels, with greater thicknesses correlating to lower pH values. Maximum pH values were reached between days 12-16 after which a decline was noted in each group, indicating a replenishing of medicament may be necessary to maintain pH levels at the external root surface.
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    Will Topical Application of Alendronate Disodium to Dental Roots Reduce Osteoclast Adherence and Resorption in Vitro?
    (2016-08) Finley, Rhett
    Introduction: The purposes of this study were: 1. To demonstrate the presence of fully differentiated, multinucleated human osteoclasts on bovine and human dentin and cementum in vitro. 2. To comparatively quantify osteoclast presence and distribution on tooth sections and adjacent lab wells following differentiation. 3. To comparatively quantify the amount of resorption that occurs when human root sections are subjected to a bisphosphonate antiresorptive treatment in comparison to no treatment. Materials and Methods: 30 sections of human tooth root and 48 sections of bovine tooth root were created using an IsoMet saw. The tooth sections from both groups were then divided into 2 equal groups of 15 and 24 sections respectively. One group received no treatment and the second group was soaked in 2mM Alendronate Disodium prior to transfer to culture. All sections were then plated in sterile culture wells with osteoclast precursor cells derived from whole human blood. The sections remained in culture until completion of osteoclastic differentiation, which took approximately 24 days. The supernatant was recovered from each well and used in an ELISA to determine the CTX-1 breakdown products from each section. The sections were fixed in paraformaldehyde and TRAP stained for visualization of osteoclasts. TRAP+ cell counts were performed on tooth sections and residual treatment wells. Student t-tests were used to determine differences between groups Results: Osteoclast attachment was observed on all human and bovine samples with the exception of one bacterially contaminated sample. The no treatment group had significantly more TRAP+ cells than the ALN group on both the human and bovine tooth sections. The no treatment group also had significantly more cells per residual well than the ALN group. No evidence of osteoclastic resorption could be demonstrated with the ELISA. Conclusions: Within the limitations of this study, it can be concluded that: 1. Fully differentiated, multinucleated osteoclasts can be successfully isolated from whole human blood and seeded onto human tooth sections in vitro. 2. Topical application of ALN disodium to extracted and sectioned tooth surfaces suppresses osteoclastic proliferation and differentiation in vitro. 3. Bovine teeth and human teeth allow for similar human osteoclast attachment and distribution in an in vitro model. 4. Mononuclear osteoclasts do not exhibit appreciable resorptive potential on sectioned surfaces in vitro.