Browsing by Subject "Discovery"
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Item Discovery(2016-05) Reim, Carl PThis story map walks readers through the routes taken by the British National Antarctic Expedition (1901-1904), commonly referred to as the Discovery Expedition. Led by Captain Robert Falcon Scott, the party spent two winters on Ross Island from which they led many scientific and exploratory excursions. Based on charts created by members of the expedition, this story map outlines the extent of the expedition as they traveled to, from and across the Frozen Continent. Additional media sources are used to help viewers walk in the steps of some of the earliest Antarctic explorers.Item Discovery, Dereplication, And Functional Assignment Of Natural Products In Streptomyces Coelicolor M145(2021-11) Marshall, AndrewNatural products are structurally complex molecules produced from numerous organisms such as fungi, plants, and bacteria. Sought after primarily for their therapeutic properties, especially as antibacterial agents, screening of natural product-producing organisms extracts from 1920-1960s resulted in a “golden age” of antibiotic discovery. However, this did not last as antibacterial resistance and rediscovery of known molecules became rampant. Re-invigoration of natural product screening efforts occurred as genome sequencing became commonplace and the biosynthetic potential of natural product producers was revealed to be much greater than previously thought. This has led to new efforts to exploit their biosynthetic potential. Key improvements in analytical tools, namely mass spectrometry, has accelerated efforts to sensitively examine natural product mixtures. Additionally, computational tools have enabled the analysis of the large datasets that are a result of these sensitive analytical tools, including quantitation and identification of many molecules, providing a more comprehensive view of natural product extracts than ever before. Application of these tools towards discovery and functional annotation of natural products enables greater insight into the production of these important molecules. This thesis is focused on developing and applying modern mass spectrometry-based approaches to investigate the natural products produced from Streptomyces coelicolor M145, the most well-studied streptomycete. First, ion mobility mass spectrometry is investigated and shown to be an effective approach to separate and distinguish isobaric cyclic-prodiginines that are produced by S. coelicolor. Second, discovery and characterization of a novel actinorhodin-related molecule, θ-actinorhodin, is accomplished using a combined metabolomics and proteomics approach. This represents the first known trimeric benzoisochromanequinone natural product and illustrates the effective use of metabolomics and proteomics on the same liquid chromatography-mass spectrometer. Third, a comprehensive analysis of the time-resolved relationship between gene expression and metabolite abundance of S. coelicolor is enabled by the use of mass spectrometry-based tools and correlation network analysis. This resulted in important conclusions about the concerted expression of biosynthetic gene clusters and the resulting production of the linked metabolite. Finally, several strategies are presented for future analysis of natural product extracts including the use of proteomics to identify putative Streptomyces using a collated list of natural product synthases and an approach combining modern dereplication tools and chemoselective probes for prioritization of natural products for purification.Item Optimizing the learning of a selected gymnastics activity.(2012-07) Almulla, SaqerThis research is testing for a new technology (Dartfish) against the traditional proprioceptive coach-assisted feedback and simple video feedback. Also, it is testing for the differences between the traditional proprioceptive feedback and imitation learning. Three groups participated in this study; traditional proprioceptive feedback (TRD), simple video feedback (VID), and Dartfish (DRF). Four tests were conducted; Pretest, Post-test-1, Post-test-2, and Retention. The task was Pommel Horse Circles (PHC), and training lasted for four weeks. Six certified gymnastics judges evaluated the performance. The results of the study indicated that the DRF group performed and retained the performance better than the TRD and VID groups. On the other hand, the results indicated that the VID group is better than the TRD group during the performance phase, but not on the retention of the skill. Imitation learning using simple video feedback is not different than the traditional coach-assisted feedback technique in regards to retaining a gymnastics skill.