Browsing by Subject "Department of Radiology"
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Item NMR Metabolomic Analysis of Biomarkers for Mouse MC3T3 Osteoblastic Cells Exposed to Ionizing Radiation(2011-04-13) Kim, HeeEunThe research is relevant to radiation therapy for bone cancer. This study seeks to determine if radiation damage to bone is observable via NMR spectroscopy of cell metabolomics. Sample preparation, NMR spectroscopic techniques and analysis were developed during the previous UROP study of osteoblastcells differentiation. We hypothesize that radiation dose and time following irradiation will influence the in vitro phenotypic expression of pre osteoblasticcells. We anticipate that in vitro radiation doses 0 and 10Gy will result in a increase in the appearance of adipocytes with a related decline in osteoblasticcells. 1D-1H NMR (700MHz) spectra of water soluble extracts from MC3T3 Osteoblasticcells were obtained. The metabolomicsignature of osteoblastcells, irradiated and control , were compared through 1D-1H NMR spectroscopy . 1H NMR Spectra was analyzed by Chenomx6.1 software which is available at the University of Minnesota Biomedical NMR facility. Chenomxdeconvolvesthe chemical shifts of an NMR spectrum and assigns them to known metabolite signatures. This allows the identification of roughly 100 to 200 metabolites. The techniques developed here will be used in future experiments for cells that are magnetically levitated to observe changes in cell metabolomicswhen gravitational loading is reduced or eliminated.Item NMR Metabolomic Analysis of Osteoblasts Pre and Post Differentiation(2010-04-21) Kim, Hee EunThe research is relevant to 18 million Americans, primarily the elderly, who suffer from osteopenia (loss of bone mass) and osteoporosis. The other targeted population is astronauts, in low-earth orbit, who lose 1 to 3 % of their bone mass per month. 1H-1DNMR (700MHz) spectra of water soluble extracts from MC3T3 Osteoblastic cells were obtained. Two osteoblast populations were studied – one cultured in cell media with the addition of ascorbic acid and the other population cultured in cell media without the addition of ascorbic. Ascorbic acid induces osteoblast cells to differentiate resulting in the production of bone. The metabolomic signature of osteoblast cells, pre and post differentiation, were compared through 1H-1DNMR spectroscopy . 1H NMR Spectra was analyzed by Chenomx 6.1 software which is available at the University of Minnesota Biomedical NMR facility. Chenomx deconvolves the chemical shifts of an NMR spectrum and assigns them to known metabolite signatures. This allows the identification of roughly 100 to 200 metabolites. Chemical shifts that Chenomx did not have in its library was assigned using 1H-2D-NOSEY NMR spectroscopy. The techniques developed here will be used in future experiments for cells that are magnetically levitated to observe changes in cell metabolomics when gravitational loading is reduced or eliminated.