Between Dec 19, 2024 and Jan 2, 2025, datasets can be submitted to DRUM but will not be processed until after the break. Staff will not be available to answer email during this period, and will not be able to provide DOIs until after Jan 2. If you are in need of a DOI during this period, consider Dryad or OpenICPSR. Submission responses to the UDC may also be delayed during this time.

Browsing by Subject "Department of Cardiology"

Now showing 1 - 1 of 1
  • Thumbnail Image
    Item
    A fast and efficient method to generate contracting embryoid bodies from murine iPs cells
    (2012-04-18) Chen, Denise
    This project aimed to test and refine a method of differentiating iPs cells into contracting embryoid bodies. Embryoid bodies (EBs) are spheroid clumps of pluripotent cells that can differentiate into various types of cells5. Spontaneous contraction of EBs indicates development into cardiomyocytes. The ability to differentiate cardiomyocytes from iPs cells can then aid in the process of cardiomyocyte regeneration for individuals who have suffered significant and damaging cardiac events. Our research aimed to develop a method of quickly and efficiently differentiating murine iPs cells into contracting EBs. Our protocol yielded areas of spontaneously contracting proportions of EBs within six days, and the peak proportion of contracting EBs was observed within nine to ten days. Further research into this topic may look into the role of norepinephrine in successfully developing EBs, or the applicability of this method of differentiation on human iPs cells.