Browsing by Subject "Cross linking"

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    Effect of extraction conditions and oxidative cross-linking on the chemical structure and microbial fermentability of arabinoxylans
    (2013-06) McClatchey, Bridget Colleen
    The aim of this thesis project was to obtain corn bran AX of varying structure by using different extraction conditions, oxidatively cross-link them, and determine the microbial fermentability of both the non-cross-linked and cross-linked samples. To reach this goal the following objectives were defined: *Determine the influence of NaOH concentration used for AX extraction on structural characteristics of extracted AX. *Determine the influence of a post extraction procedure, ball milling, on the structural characteristics of extracted AX. *Cross-link differently extracted AX by using laccase and oxygen. *Determine the effects of ferulate cross-linking on fermentability in an in vitro fermentation experiment and analyze fermentation end products (pH, gas volume, and SCFAs). *Determine the release/formation of phenolic metabolites by gut microorganisms as well as the percentage of original FA being metabolized by gut microbiota.