Browsing by Subject "Cell Culture"

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    Assessing Raw 264.7 migration in a mechanically-tunable 3D collagen-hyaluronic acid co-gel for use in Borrelia Burgdorferi pathology study
    (2024-05) Tucker, Jared
    Borrelia Burgdorferi, the bacteria associated with Lyme Disease, has been a topic of much research in recent decades, but it continues to prove difficult to understand how the bacteria interacts with the immune system on a cellular level, specifically macrophages. To aid in this work, a 3D hydrogel for cell culture was formulated using collagen and hyaluronic acid (HA) to help provide a system for studying these dynamic cellular responses in a soft-tissue environment. A design of experiments was organized to study the effects of collagen concentration, HA fragment size, and Collagen:HA mass ratio on both the hydrogel properties and macrophage mobility and growth. Mechanical characterization of the gels was conducted using rheology and found that collagen concentration, but not HA content, directly modulated the hydrogel storage modulus. The hydrogels were shown to be suitable for cell culture, allowing for cellular proliferation, but exhibited limited chemotaxis.
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    Modeling the Effects of Small Molecule Therapeutics on Glycolysis and Lactate Flux
    (2018-09) Schroeder, Joseph S; O'Brien, Conor M; Hu, Wei-Shou
    Chinese Hamster Ovary (CHO) cells are widely used in the industrial production of commercial therapeutics. One key aspect to the productivity of these cells is their high rate of glucose consumption. The high rate of glucose consumption is paired with a high output of lactate which can lead to negative culture performance. The rate of glucose consumption and lactate production can be modulated by a number of chemicals, some of which are being explored as therapeutic drugs, which affect the activities of the enzymes involved in glucose metabolism. This research aimed to evaluate the effects caused by small molecule therapeutics on CHO cells’ metabolism using a mathematic model of glucose metabolism. To model the therapeutics, established kinetic information for these therapeutics was implemented into a metabolic model. Then different concentrations of therapeutics were explored to assess their effects on metabolism. In addition, combinations of therapeutics were examined to study the effects of more drastic changes to metabolism. These therapeutics showed a large impact to the bistability of glucose metabolism as well as the lactate flux. These outcomes were important due to the potential to increase the productivity of CHO cells for industrial use as well as decreasing cell death. Thus, these therapeutics could be used to reduce lactate production in cells allowing for higher productivity.