Between Dec 19, 2024 and Jan 2, 2025, datasets can be submitted to DRUM but will not be processed until after the break. Staff will not be available to answer email during this period, and will not be able to provide DOIs until after Jan 2. If you are in need of a DOI during this period, consider Dryad or OpenICPSR. Submission responses to the UDC may also be delayed during this time.

Browsing by Subject "Beta-like cells"

Now showing 1 - 1 of 1
  • Thumbnail Image
    Item
    Determination of Methods for Improved Reprogramming of Various Cell Types to Functional Beta-like Cells
    (2016-12) Sripathy Rao, Srinidhi Rao
    Reprogramming is the process of converting a somatic cell into a different cell type in a controlled manner without an intermediate pluripotent state, usually by expression of exogenous transcription factors. Here, 3 transcription factors, (Pdx1, Ngn3 and MafA) incorporated in a polycistronic adenoviral vector system (Ad-PNM) was used to directly reprogram cells to beta-like cells that produce insulin in vitro. In addition, the role of innate immunity activation and open chromatin structure during reprogramming is studied to improve overall reprogramming efficiency while using the Ad-PNM adenoviral vector.