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Item Growth performance, carcass characteristics, physiological and gut health effects of feeding diets containing bacitracin methylene disalicylate (BMD) to heat-stressed finishing pigs.(2009-12) Song, RanHeat stress affects pig growth performance, carcass composition, and immune status. The present study was conducted to evaluate the effects of heat stress and adding bacitracin methylene disalicylate (BMD, 30g/ton) to a 10% DDGS commercial diet on growth performance, carcass characteristics, physiological parameters, small intestine morphology, and hindgut volatile fatty acid (VFA) production of finishing pigs. Four groups of 32 finishing pigs (n = 128) with initial BW between 80 to 90 kg were used in this study. Pigs were randomly assigned to diets and environmental temperature treatments in a 2 x 2 factorial arrangement. Pigs were fed a control (CON) or BMD (30g/ton) diet and exposed to a constant thermal neutral temperature (23°C) or cyclical heat stress conditions (37°C from 10:00 to 19:00 and 27°C from 19:00 to 10:00) in environmental chambers for a 28-d experimental period. Pigs housed under heat stress conditions had significantly lower average daily gain (ADG, P < 0.0001), average daily feed intake (ADFI, P < 0.0001), gain:feed (G:F, P < 0.001), and higher average daily water intake (ADWI, P = 0.03), compared with pigs housed in the thermoneutral environment. Supplementation of BMD in the diet did not improve growth performance of pigs. Average daily gain tended (P = 0.07) to be lower for pigs fed the BMD diet, while ADFI, G:F, and ADWI were not affected by dietary treatment. Carcass characteristics did not differ between dietary treatments. However, pigs assigned to the heat stress environment had lower live BW (P < 0.0001) and lower hot carcass weight (P < 0.0001) than pigs housed in the thermal neutral environment. Dressing %, 10th rib back fat depth, loin eye area, and lean % were not affected by temperature treatment. Saliva cortisol concentration did not differ between dietary treatments during the experimental period, but the initial level was lower (P < 0.05) for pigs fed the BMD diet. Heat stress led to an increased (P < 0.05) level of saliva cortisol on d 1, but no effects were observed on the following days. Serum haptoglobin concentration was not different between dietary treatments, while heat stressed pigs showed a higher (P < 0.05) level of haptoglobin on d 1, and levels tended to remain higher (P < 0.1) on d 13 of heat stress. Cytokines IL-1β and TNF-α were not affected by heat stress, but pigs fed the BMD diet had an initial lower (P < 0.0001) level of serum IL-1β, and tended to be lower (P < 0.1) on d 13 of heat stress as compared to pigs fed CON. Small intestine morphology was not affected by temperature treatment, but pigs fed the BMD diet tended to have greater (P = 0.07) villi height at duodenum, and greater crypt depth at duodenum (P = 0.09) and jejunum (P = 0.07), respectively. Dietary treatment did not affect VFA production in the cecum, while pigs housed under heat stress conditions tended to have less propionate (P = 0.08) concentration, greater A:P ratio (P = 0.08), and significantly less valerate (P = 0.02) produced in the cecum compared with pigs under the thermoneutral environment.