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Browsing by Author "Wang, Chun"

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    Assessment of Polymer Wafer Aided Gene Transfection Efficiency and Cell Viability
    (2018-08) Nathan, Noah; Hanson, Samuel; Wang, Chun
    DNA transfection of eukaryotic cells has been explored using non-chemical, chemical and viral methods. Mucoadhesive, water-soluble and neutral polymer wafers offer a novel sublingual DNA delivery method. Transfection efficiency and viability of GFP/PEI polyplexes in various polymer delivery vehicles into murine fibroblasts (NIH3T3) was studied using flow cytometry, fluorescence microscopy and MTT colorimetric assay. Polyvinyl alcohol (PVA) and hydroxypropyl cellulose (HPC) wafers in HEPES buffer had an increased transfection efficiency compared to polyplexes delivered in HEPES buffer only. DNA/PEI polyplexes delivered in PVA or polyethylene oxide (PEO or PEG) had the highest viability of all the transfection methods.