Browsing by Author "Sheats, Julian"
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Item Data from: Distribution of distances between DNA barcode labels in nanochannels close to the persistence length(2015-02-14) Reinhart, Wesley F; Reifenberger, Jeff G.; Gupta, Damini; Muralidhar, Abhiram; Sheats, Julian; Cao, Han; Dorfman, Kevin D.Item Data from: Main text figure data for Subdiffusion of loci and cytoplasmic particles are different in compressed Escherichia coli cells(2018) Yu, Shi; Sheats, Julian; Sclavi, Bianca; Cicuta, Pietro; Cosentino Lagomarsino, Marco; Dorfman, Kevin DItem Data from: Measurements of DNA barcode label separations in nanochannels from time-series data(2015) Sheats, Julian; Reifenberger, Jeff G.; Cao, Han; Dorfman, Kevin D.Item Data from: Mixed Confinement Regimes during Equilibrium Confinement Spectroscopy of DNA(2014-06-04) Gupta, Damini; Sheats, Julian; Muralidhar, Abhiram; Miller, Jeremy J.; Huang, Derek E.; Mahshid, Sara; Dorfman, Kevin D.; Reisner, WalterItem Data from: Modeling the relaxation of internal DNA segments during genome mapping in nanochannels(2016) Jain, Aashish; Sheats, Julian; Reifenberger, Jeffery G; Cao, Han; Dorfman, Kevin DItem Data Repository for "Role of Growth Rate on the Orientational Alignment of E. coli in a Slit"(2017) Sheats, Julian; Sclavi, Bianca; Cicuta, Pietro; Cosentino Lagomarsino, Marco; Dorfman, Kevin DItem Data supporting 'Subdiffusion of loci and cytoplasmic particles are different in compressed E. coli cells'(2018-05-15) Yu, Shi; Sheats, Julian; Cicuta, Pietro; Sclavi, Bianca; Cosentino Lagomarsino, Marco; Dorfman, Kevin D; dorfman@umn.edu; Dorfman, Kevin DThe complex physical nature of the bacterial intracellular environment remains largely unknown, and has relevance for key biochemical and biological processes of the cell. While recent work has addressed the role of non-equilibrium drives and crowding, the consequences of mechanical perturbations are relatively less explored.We have used a microfabricated valve system to track both fluorescently labeled chromosomal loci and cytoplasmic particles in E.~coli cells shortly after the application of a compressive force on time scales that are too sudden to allow for biochemical response from the cell. While cytoplasmic diffusion is slowed down significantly under compression, the mobility of DNA loci is much less affected. These results suggest that the dynamics of the bacterial chromosome are decoupled from the viscoelastic environment of the cytoplasm under such short time scales, and that DNA elasticity and nucleoid organization play a more important role in loci subdiffusion than cytoplasmic viscoelasticity.