Browsing by Author "Rendahl, Aaron"
Now showing 1 - 8 of 8
Results Per Page
Sort Options
Item Anti-CAR antibody response in SIV infected rhesus macaques(2022-11-28) Davey, Brianna, C; Pampusch, Mary, S; Cartwright, Emily, K; Abdelaal, Hadia, M; Rakasz, Eva, G; Rendahl, Aaron; Berger, Edward, A; Skinner, Pamela, J; skinn002@umn.edu; Skinner, Pamela, J; Skinner laboratoryT cells expressing a simian immunodeficiency (SIV)-specific chimeric antigen receptor (CAR) and the follicular homing molecule, CXCR5, were infused into antiretroviral therapy (ART) suppressed, SIV-infected rhesus macaques to assess their ability to localize to the lymphoid follicle and control the virus upon ART interruption. The cells did not persist in the animals beyond 28 days. Development of anti-CAR antibodies could be responsible for the lack of persistence. Potential antigenic sites on the anti-SIV CAR used in these studies included domains 1 and 2 of CD4, the carbohydrate recognition domain (CRD) of mannose-binding lectin (MBL), and an extracellular domain of the costimulatory molecule, CD28, along with short linker sequences. Using a flow cytometry based assay and target cells expressing the CAR/CXCR5 construct, we examined the serum of the CAR T-cell treated animals to determine that the animals had developed an anti-CAR antibody response after infusion. Binding sites for the anti-CAR antibodies were identified by using alternative CARs transduced into target cells and by preincubation of the target cells with a CD4 blocking antibody. All of the treated animals developed antibodies in their serum that bound to CAR-T cells and the majority were capable of inducing an ADCC response. The CD4 antibody-blocking assay suggests that the dominant immunogenic components of this CAR are the CD4 domains with a possible additional site of the CD28 domain with its linker.Item CT radiomic features for canine primary lung tumors(2021-07-20) Wolf-Ringwall, Amber; Rendahl, Aaron; Seelig, Davis; Ober, Chris; Able, Hannah; Wilke, Chris; Lawrence, Jessica; jlawrenc@umn.edu; Lawrence, Jessica; University of Minnesota Veterinary OncologyQuantitative analysis of computed tomography (CT) radiomic features is an indirect measure of tumor heterogeneity, which has been associated with prognosis in human lung carcinoma. Canine lung tumors share similar features to human lung tumors and may serve as a model in which to investigate the utility of radiomic features in differentiating tumor type and prognostication. The purpose of this study was to correlate first-order radiomic features from canine primary lung tumors to histopathologic characteristics and outcome. Sixty-seven tumors from 65 dogs were evaluated in this study.Item Estimation of Ideal Body Weight in Horses and Ponies Using Morphometric Measurements(University of Minnesota. Minnesota Extension Service, 2012-09) Martinson, Krishona; Coleman, Robert; Earing, Jennifer; Rendahl, Aaron; McCue, MollyItem Identification of Student Lifestyle Characteristics Associated with Training Choices to Drive Targeted Admissions in Veterinary Medicine(Education in the Health Professions, 2020) Root Kustritz, Margaret V; Malone, Erin; Rendahl, AaronBackground: There is an identified need for practicing veterinarians with a focus on food animal work in the United States. Students at the University of Minnesota College of Veterinary Medicine track by species (food animal, equine, mixed, and small animal) or discipline (research) in the latter part of their training. Identification of life experiences that are associated with students choosing the food animal track would permit the college to better target admissions to meet societal needs. Aims and Objectives: To identify lifestyle characteristics and activities associated with choice of the food animal track and to evaluate how student choice of track varies across their training. Materials and Methods: Students from three consecutive classes were surveyed to identify factors influencing track choice. Fisher’s test was used to compare data and Clopper–Pearson “exact” confidence intervals computed. Results: Students who declared interest in the 1st year in small animal, equine, or food animal as a track were highly likely to choose that at their final track later in the curriculum. Eightyfive percent of students in a food animalfocused early admission track chose the food animal track; the remainder chose the mixed track with cattle as one of their species of interest. Students were more likely to choose the food animal track if their undergraduate major was animal science, if they grew up in a rural area, lived on a farm, were in 4H or were in Future Farmers of America, or had shown or worked horses or cattle, or shadowed a large animal veterinarian. Students valued mentoring from within the college and from outside veterinarians. Conclusions: Knowledge of how students choose their tracks will permit the college better to promote admissions of students who are more likely to track food animal and to plan for adequate clinical year experiences for all students.Item Metabolomics reveals the origins of antimicrobial plant resins collected by honey bees(Public Library of Science, 2013) Wilson, Michael B.; Spivak, Marla; Hegeman, Adrian D.; Rendahl, Aaron; Cohen, Jerry D.The deposition of antimicrobial plant resins in honey bee, Apis mellifera, nests has important physiological benefits. Resin foraging is difficult to approach experimentally because resin composition is highly variable among and between plant families, the environmental and plant-genotypic effects on resins are unknown, and resin foragers are relatively rare and often forage in unobservable tree canopies. Subsequently, little is known about the botanical origins of resins in many regions or the benefits of specific resins to bees. We used metabolomic methods as a type of environmental forensics to track individual resin forager behavior through comparisons of global resin metabolite patterns. The resin from the corbiculae of a single bee was sufficient to identify that resin’s botanical source without prior knowledge of resin composition. Bees from our apiary discriminately foraged for resin from eastern cottonwood (Populus deltoides), and balsam poplar (P. balsamifera) among many available, even closely related, resinous plants. Cottonwood and balsam poplar resin composition did not show significant seasonal or regional changes in composition. Metabolomic analysis of resin from 6 North American Populus spp. and 5 hybrids revealed peaks characteristic to taxonomic nodes within Populus, while antimicrobial analysis revealed that resin from different species varied in inhibition of the bee bacterial pathogen, Paenibacillus larvae. We conclude that honey bees make discrete choices among many resinous plant species, even among closely related species. Bees also maintained fidelity to a single source during a foraging trip. Furthermore, the differential inhibition of P. larvae by Populus spp., thought to be preferential for resin collection in temperate regions, suggests that resins from closely related plant species many have different benefits to bees.Item Monitoring the Curriculum through the Student Perspective(Journal of Veterinary Medical Education, 2020) Malone, Erin D; Root Kustritz, Margaret V; Rendahl, Aaron; Molgaard, Laura KStudent input was deliberately included as part of the curriculum implementation and assessment plan at the University of Minnesota College of Veterinary Medicine. The new curriculum included design features to encourage deeper learning such as a spiral curriculum with cross-course integration, increased open time, and more active learning. Student well-being was seen as a simultaneous need. To gather overall perceptions of workload and well-being, student volunteers from each cohort were surveyed weekly starting in 2013. Survey questions asked about out-of-class work time, level of integration, extracurricular activities, student well-being habits, paid employment, and other factors. Survey questions were combined with course data to get a full picture of week quality, total course work time, extracurricular activities, and the effects of integration. Many of our hypotheses about curricular and extracurricular impacts on week quality were disproven. Week quality was most positively affected by student factors of sleep and exercise, whereas the curricular factors of out-of-class work time, total course work time, and examination hours had the strongest negative effects. A surprising finding was that open time, in-class hours, and paid employment hours had a minimal effect on week quality. Students identified excessively heavy semesters and uneven semester workloads that resulted in early revisions to the new curriculum. Student feedback provided a view of the curriculum that was not otherwise available and resulted in early and significant impacts on the new curriculum, and they provided insight into whether planned changes had occurred and how effective various factors were in reaching the curricular goals.Item Using a model board examination and a case study assessing clinical reasoning to evaluate curricular change(Education in the Health Professions, 2018) Root Kustritz, Margaret V; Rendahl, Aaron; Molgaard, Laura K; Malone, ErinBackground: This study compared student ability to integrate basic science and clinical information before and after implementing a curriculum revision that introduced a problem‑oriented case approach as required coursework. Materials and Methods: Student knowledge and competence were assessed just before entry into clinical training by completion of 100 multiple‑choice questions mirroring the breadth and type of questions on the national licensing examination (Part I) and by completion of 10 cases to discern clinical decision‑making (Part II). Scores from students from the classes of 2015 and 2016 (previous curriculum) were compared to those from students from the classes of 2017 and 2018 (current curriculum). Results: Part I scores were not significantly different between any classes in the previous and current curriculum. Part II scores for 3rd‑year students in the current curriculum were higher than those for comparable students in the past 2 years of the previous curriculum. Mean scores for the class of 2016, the last year of the previous curriculum, were significantly lower than all other classes. Conclusion: Students benefit from measured and repetitive practice in clinical reasoning.Item Using a model board examination and a case study assessing clinical reasoning to evaluate curricular change(Education in the Health Professions, 2018) Root Kustritz, Margaret V; Rendahl, Aaron; Molgaard, Laura; Malone, ErinBackground: This study compared student ability to integrate basic science and clinical information before and after implementing a curriculum revision that introduced a problem-oriented case approach as required coursework. Materials and Methods: Student knowledge and competence were assessed just before entry into clinical training by completion of 100 multiple-choice questions mirroring the breadth and type of questions on the national licensing examination (Part I) and by completion of 10 cases to discern clinical decision-making (Part II). Scores from students from the classes of 2015 and 2016 (previous curriculum) were compared to those from students from the classes of 2017 and 2018 (current curriculum). Results: Part I scores were not significantly different between any classes in the previous and current curriculum. Part II scores for 3rd-year students in the current curriculum were higher than those for comparable students in the past 2 years of the previous curriculum. Mean scores for the class of 2016, the last year of the previous curriculum, were significantly lower than all other classes. Conclusion: Students benefit from measured and repetitive practice in clinical reasoning.