Browsing by Author "Neitzke, Colin C"

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    Development of Fibrinogen-based Bioinks for 3D Bioprinting Motor Neuron Progenitor Cells
    (2017-08-23) Neitzke, Colin C
    In the United States, there are more than 12,000 new cases of spinal cord injury each year. No effective treatment is available for these injuries except high-expense lifetime care. Here we introduce a fibrinogen-based bioink for the purpose of 3D bioprinting motor neuron progenitor cells (MNPCs) into hydrogel scaffolds as a potential treatment for major spinal cord injury. While other bioinks are commercially available, such as gelatin methylacrylate (GELMA) and Matrigel, a murine Engelbreth-Holm-Swarm (EHS) sarcoma extract, these bioinks are toxic to MNPCs and are not fully defined, respectively. For this reason, a fully defined bioink that is not toxic to MNPCs must be developed. The results suggest that the bioink developed for 3D bioprinting supports viability and differentiation of MNPCs, but the printing process that MNPCs go through while being extruded into hydrogel scaffolds must further be optimized to increase MNPC viability and differentiation.
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    Supporting data for 3D Printed Stem-Cell Derived Neural Progenitors Generate Spinal Cord Scaffolds
    (2020-05-15) Joung, Daeha; Truong, Vincent; Neitzke, Colin C; Guo, Shuang-Zhuang; Walsh, Patrick J; Monat, Joseph R; Meng, Fanben; Park, Sung Hyun; Dutton, James R; Parr, Ann M; McAlpine, Michael C; mcalpine@umn.edu; McAlpine, Michael C; McAlpine Research Group
    A bioengineered spinal cord is fabricated via extrusion-based multilateral 3D bioprinting, in which clusters of induced pluripotent stem cell (iPSC)-derived spinal neuronal progenitor cells (sNPCs) and oligodendrocyte progenitor cells (OPCs) are placed in precise positions within 3D printed biocompatible scaffolds during assembly. The location of a cluster of cells, of a single type or multiple types, is controlled using a point-dispensing printing method with a 200 μm center-to-center spacing within 150 μm wide channels. The bioprinted sNPCs differentiate and extend axons throughout microscale scaffold channels, and the activity of these neuronal networks is confirmed by physiological spontaneous calcium flux studies. Successful bioprinting of OPCs in combination with sNPCs demonstrates a multicellular neural tissue engineering approach, where the ability to direct the patterning and combination of transplanted neuronal and glial cells can be beneficial in rebuilding functional axonal connections across areas of central nervous system (CNS) tissue damage. This platform can be used to prepare novel biomimetic, hydrogel-based scaffolds modeling complex CNS tissue architecture in vitro and harnessed to develop new clinical approaches to treat neurological diseases, including spinal cord injury.